PGAM2 Mouse mAb - Primärer Antikörper, specific to PGAM2

Cat. No.: Ab121443

GRADE & PURITY Validated, ExactAb™, See COA

Application

IF/ICC, WB

Host species

Maus

Reactive species

Affe, Allerdings, Maus, Mensch, Rat

Isotype

Mouse IgG2a

Clone Number

OTI4B12

Conjugation

Unconjugated

Übersicht

Mouse anti Human PGAM2 Antibody, Monoclonal (OTI4B12), could be used for WB, IF/ICC and so on.

Specifications

Product Name

PGAM2 Mouse mAb - Primärer Antikörper, specific to PGAM2

Synonyme

BPG dependent PGAM 2 antibody | BPG-dependent PGAM 2 antibody | GSD10 antibody | MGC88743 antibody | Muscle specific phosphoglycerate mutase antibody | Muscle-specific phosphoglycerate mutase antibody | OTTHUMP00000207787 antibody | PGAM 2 antibody | PGAM

Spezifikationen & Reinheit

ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Wirtsspezies

Maus

Spezifität

PGAM2

Immunogen

Recombinant full length protein of human PGAM2.

Positive Kontrolle

WB: Hep G2, HeLa, Jurkat, MCF7, A431, NIH/3T3 and C6 cell lysates. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY PGAM2.

Konjugation

Unconjugated

Note

ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: PGAM2 Mouse mAb (Ab121443)

PGAM2 Mouse mAb (Ab121443) - Western Blot
All lanes: PGAM2 Mouse mAb (Ab121443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 27 kDa
Exposure time: 3.1 s

IF/ICC: PGAM2 Mouse mAb (Ab121443)

PGAM2 Mouse mAb (Ab121443) - IF/ICC
PGAM2 Mouse mAb (Ab121443) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/100;

Zugehörige Ziele (menschlich)

PGAM2 Tbio Phosphoglycerate mutase 2 (0 Activities)

Discover Target: PGAM2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PGAM2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.