GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Proteomics grade?Proteomics grade — ultra-clean, low-contaminant reagents for proteomic MS workflows. Use in sample prep for protein identification and quantitation.≥95%(SDS-PAGE)
BioReagent, Proteomics grade, ≥95%(SDS-PAGE) BioReagent,Proteomics grade for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.
Application:
PNGase F from Elizabethkingia meningoseptica has been used:
for de-N-glycosylation of Zika20virus E protein
to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)
Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
Used to deglycosylate protein.
Specifications
Product Name
PNGase F from Elizabethkingia meningoseptica, CAS No.83534-39-8
Grade
BioReagent, Proteomics grade
Specifications & Purity
BioReagent, Proteomics grade, ≥95%(SDS-PAGE)
Biochemical and Physiological Mechanisms
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
CAS
83534-39-8
Enzyme Commission Number
3.5.1.52(BRENDA, IUBMB)
Molecule Type
Enzyme
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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