Profilin 1 Mouse mAb - Primärer Antikörper, specific to Profilin 1, Mouse IgG2b

Cat. No.: Ab191887
Zu bestellen verfügbar
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Flow Cytometry, IF/ICC, WB
Host species
Maus
Reactive species
Mensch
Isotype
Mouse IgG2b
Clone Number
AB01/2E11
Conjugation
Unconjugated
Size
Deutschland (EU)
USA*
Price
Qty
10μl
Ab191887-10μl
Auf Lager ≥10
86,69€
50μl
Ab191887-50μl
Auf Lager ≥10
225,53€
100μl
Ab191887-100μl
5 Auf Lager
364,36€
1ml
Ab191887-1ml
Auf Bestellung · 8–12 Wochen
2.950,23€
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Übersicht

Mouse anti Human Profilin 1 Antibody, clone AB01/2E11, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name
Profilin 1 Mouse mAb - Primärer Antikörper, specific to Profilin 1, Mouse IgG2b
Synonyme
Actin binding protein antibody | ALS18 antibody | Epididymis tissue protein Li 184a antibody | OTTHUMP00000125244 antibody | PFN 1 antibody | Pfn antibody | PFN1 antibody | PROF1_HUMAN antibody | Profilin I antibody | Profilin-1 antibody | ProfilinI antib
Spezifikationen & Reinheit
Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
Biochemische und physiologische Mechanismen
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androg
Wirtsspezies
Maus
Spezifität
Profilin 1
Immunogen
Recombinant protein corresponding to Human Profilin 1
Positive Kontrolle
WB: HeLa, HepG2, HT-29, K562, HEK293 and Jurkat whole cell lysates Flow: HeLa cells IF/ICC: U-2 OS cells
Konjugation
Unconjugated
Note
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotyp
Mouse IgG2b
Lichterketten-Typ
lambda
SDS-PAGE
150.0 KDa
Verfahren zur Reinigung
Protein A purified
Konzentration
1.0 mg/mL
Lagerung und Versand
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Bilder

Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s

Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s

Profilin 1 Mouse mAb (Ab191887) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Profilin 1 Mouse mAb (Ab191887) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).

Profilin 1 Mouse mAb (Ab191887) - ICC/IF
IF analysis of Profilin 1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Profilin 1 Mouse mAb (Ab191887) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Anwendung
AnwendungDilution info
IF/ICC

1/200

WB

1/1,000 - 1/100,000; Predicted molecular weight: 15.0 KDa

Flow Cytometry

1/200 - 1/25000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Zugehörige Ziele (menschlich)
PFN1 Tbio Profilin-1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Zertifikate (CoA, COO, BSE/TSE und Analyse-Diagramm)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDatumArtikel
ZJ24F0910975Certificate of AnalysisJul 10, 2026 Ab191887
ZJ24F0910976Certificate of AnalysisJul 10, 2026 Ab191887
ZJ24F0910977Certificate of AnalysisJul 10, 2026 Ab191887
ZJ24F0910978Certificate of AnalysisJul 10, 2026 Ab191887
FAQs und Artikel
Lösungsrechner
Bewertungen

Kundenbewertungen

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Animal Free grade guide → View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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