PSMD1 Mouse mAb - Primary antibody, specific to PSMD1, Mouse IgG1

Cat. No.: Ab123682

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

K70027_8C11

Conjugation

Unconjugated

Overview

Mouse anti Human PSMD1 Antibody, Monoclonal (K70027_8C11), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/200 
Protein Function

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

Specifications

Product Name

PSMD1 Mouse mAb - Primary antibody, specific to PSMD1, Mouse IgG1

Synonyms

MGC133041 antibody | 26S proteasome non ATPase regulatory subunit 1 antibody | 26S proteasome non ATPase subunit 1 antibody | 26S proteasome non-ATPase regulatory subunit 1 antibody | 26S proteasome regulatory subunit rpn2 antibody | 26S proteasome regula

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Host species

Mouse

Specificity

PSMD1

Immunogen

Recombinant protein of human PSMD1 (AA 791-920).

Positive Control

WB: Hep G2, HeLa, Jurkat, MCF7, A431, SK-OV-3, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PSMD1 Mouse mAb (Ab123682)

PSMD1 Mouse mAb (Ab123682) - Western Blot
All lanes: PSMD1 Mouse mAb (Ab123682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 108 kDa
Exposure time: 45.7 s

IHC: PSMD1 Mouse mAb (Ab123682)

PSMD1 Mouse mAb (Ab123682) - IHC
IHC-P analysis of testis tissue by PSMD1 Mouse mAb (Ab123682).IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PSMD1 Mouse mAb (Ab123682) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the nuclei and cytoplasm.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/200 ;

Associated Targets(Human)

PSMD1 Tbio 26S proteasome non-ATPase regulatory subunit 1 (0 Activities)

Discover Target: PSMD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action