RBCK1 Mouse mAb - Primärer Antikörper, specific to RBCK1, Mouse IgG1

Cat. No.: Ab124756

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

K39S7K

Conjugation

Unconjugated

Übersicht

Mouse anti Human RBCK1 Antibody, Monoclonal (K39S7K), could be used for WB and so on.

Specifications

Product Name

RBCK1 Mouse mAb - Primärer Antikörper, specific to RBCK1, Mouse IgG1

Synonyme

Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both h

Wirtsspezies

Maus

Spezifität

RBCK1

Immunogen

Recombinant fusion protein of human RBCK1.

Positive Kontrolle

WB: Jurkat, HEK293, A375 and A549 cell lysates.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: RBCK1 Mouse mAb (Ab124756)

RBCK1 Mouse mAb (Ab124756) - Western Blot
All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117).
The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050).
Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117).
Exposure time: 65.7 s

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

Zugehörige Ziele (menschlich)

RBCK1 Tbio RanBP-type and C3HC4-type zinc finger-containing protein 1 (0 Activities)

Discover Target: RBCK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RBCK1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.