Recombinant CD19 Antibody (AF488) - Primary antibody, specific to Human CD19

Cat. No.: Ab224663
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 5 μL/test
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
P08D14
Conjugation
AF488
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10T
Ab224663-10T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$39.90
25T
Ab224663-25T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$79.90
100T
Ab224663-100T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$159.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 5 μL/test ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant CD19 Antibody, clone P08D14, could be used for Flow Cytometry.

Specifications

Product Name
Recombinant CD19 Antibody (AF488) - Primary antibody, specific to Human CD19
Synonyms
B4 | B-lymphocyte antigen CD19 | B-lymphocyte surface antigen B4 | CD19 antigen | CD19 molecule | CD19 | CVID3 | Differentiation antigen CD19 | Leu-12 | MGC12802 | T-cell surface antigen Leu-12 | Antibody deficiency due to defect in CD19 antibody | Antibo
Specifications & Purity
ExactAb™, Validated, Recombinant, 5 μL/test
Biochemical and Physiological Mechanisms
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to th
Host species
Mouse
Specificity
Human CD19
Immunogen
Human Chronic Lymphocytic Leukemia (CLL) Cells.
Positive Control
Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
Conjugation
AF488
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Recombinant CD19 Antibody (AF488) (Ab224663) - Flow Cytometry
Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD19 Antibody (AF488) (Ab224663) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

Recombinant CD19 Antibody (AF488) (Ab224663) - Flow Cytometry
Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (AF488) (Ab224663) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0825935Certificate of AnalysisJun 18, 2026 Ab224663
ZJ25F0825936Certificate of AnalysisJun 18, 2026 Ab224663
ZJ25F0825937Certificate of AnalysisJun 18, 2026 Ab224663
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