Recombinant CD45.1 Antibody (APC) - Primary antibody, specific to Mouse CD45.1

Cat. No.: Ab213891

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

P15B07

Conjugation

APC

Overview

Recombinant CD45.1 Antibody, clone P15B07, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD45.1 Antibody (APC) - Primary antibody, specific to Mouse CD45.1

Synonyms

B220 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | LY5 antibody | Protein tyrosine phosphatase receptor type C antibody | Protein tyrosine phosphatase, receptor type, c polypeptide anti

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Host species

Mouse

Specificity

Mouse CD45.1

Immunogen

SJL mouse thymocytes and splenocytes.

Positive Control

Flow Cytometry: SJL mouse splenocytes.

Conjugation

APC

Excitation (nm)

Red Laser(633nm)

Emission(nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD45.1 Antibody (APC) (Ab213891)

Recombinant CD45.1 Antibody (APC) (Ab213891) - Flow Cytometry
Flow cytometry analysis of CD45.1 expression by SJL mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45.1 Antibody (APC) (Ab213891) at 0.1μg/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309).

Flow Cytometry: Recombinant CD45.1 Antibody (APC) (Ab213891)

Recombinant CD45.1 Antibody (APC) (Ab213891) - Flow Cytometry
Flow cytometry analysis of CD45.1 expression by SJL mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45.1 Antibody (APC) (Ab213891) at 0.1μg/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)