GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥90%(SDS-PAGE)
Expression System
HEK293
Accession #
Q8TDQ0
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,His Tag,PBS Only,≥90%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Protein Function:： Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
Specifications
Product Name
Recombinant Human TIM-3 Protein
Synonyms
FLJ14428 | HAVCR2 | HAVcr-2 | hepatitis A virus cellular receptor 2 | kidney injury molecule-3 | KIM-3 | SPTCL | T cell immunoglobulin mucin 3 | CD366 | T cell immunoglobulin mucin-3 | T-cell immunoglobulin and mucin domain-containing protein 3 | TIM 3 |
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only
Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.
40.0-50.0 kDa, under reducing conditions; 48.0 & 65.0-90.0 kDa, under non-reducing conditions
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Images
Recombinant Human TIM-3 Protein (rp176703) - Binding Activity Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC₅₀ of 1.267 μg/mL.
Recombinant Human TIM-3 Protein (rp176703) - ELISA Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC₅₀ of 15.3 ng/mL.
Recombinant Human TIM-3 Protein (rp176703) - SDS-PAGE 3 μg/lane of Recombinant Human TIM-3 Protein was resolved with SDS-PAGE under reducing (R) and no-reducing (N) and visualized by Coomassie® Blue staining, showing a band at 40.0-50.0 kD under reducing conditions and 48.0 kDa and 65.0-90.0 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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