Recombinant PCNA Antibody - Primärer Antikörper, specific to PCNA, Rabbit IgG

Cat. No.: Ab120681

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC, IP, WB

Host species

Kaninchen

Reactive species

Allerdings, Grüner Affe, Huhn, Kuh, Mensch, Rat

Isotype

Rabbit IgG

Clone Number

RR704

Conjugation

Unconjugated

Übersicht

Rabbit anti Human PCNA Antibody, Recombinant (RR704), could be used for WB, Flow, IF/ICC, IP and so on.
Application:
WB: 1/1000 - 1/2000
Flow: 1/800 - 1/2000

IF/ICC: 1/1000
IP: 1/50

Specifications

Product Name

Recombinant PCNA Antibody - Primärer Antikörper, specific to PCNA, Rabbit IgG

Synonyme

ATLD2 antibody | cb16 antibody | Cyclin antibody | DNA polymerase delta auxiliary protein antibody | etID36690.10 antibody | fa28e03 antibody | fb36g03 antibody | HGCN8729 antibody | MGC8367 antibody | Mutagen-sensitive 209 protein antibody | OTTHUMP00000

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.5 mg/mL

Wirtsspezies

Kaninchen

Spezifität

PCNA

Immunogen

A synthetic peptide corresponding to residues of human PCNA was used as an immunogen (AA 100-200).

Positive Kontrolle

WB: A431, HEK293, MCF7, HeLa, Hep G2, NIH/3T3, C6, K562, Jurkat and HUVEC cell lysates. Flow: HeLa cells. IP: HeLa whole cell lysate. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.5 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution

Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 14.1 s

ICC/IF : Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant PCNA Antibody (Ab120681) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant PCNA Antibody (Ab120681) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant PCNA Antibody (Ab120681) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

IP: Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - IP
PCNA was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant PCNA Antibody (Ab120681) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant PCNA Antibody (Ab120681) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant PCNA Antibody (Ab120681) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).

Anwendung

Anwendung

Dilution info

WB

1/1000 - 1/2000

IF/ICC

1/1000

IP

1/50

Flow Cytometry

1/800 - 1/2000

Zugehörige Ziele (menschlich)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant PCNA Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.