Rennin, CAS No.9001-98-3

CAS: 9001-98-3 Cat. No.: R755308 Molecular Weight: 669.5 EC Number: 232-645-0 PubChem CID: 34231

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from calf stomach; ≥10 U/mg enzyme powder; ≥20 U/mg protein

Accession #

P00794

Bioactivity

≥10 U/mg enzyme powder; ≥20 U/mg protein

Overview

Rennin, also known as Chymosin, is a pepsin-related proteolytic enzyme synthesized by cells in the stomach of certain animals that efficiently converts liquid milk into a semi-solid, allowing it to remain in the stomach for longer. The natural substrate of Rennin is K-casein, which is specifically cleaved at the peptide bond between amino acid residues 105 and 106, phenylalanine and methionine, and is widely used in cheese production.

Specifications

Product Name

Rennin, CAS No.9001-98-3

Synonyms

Chymosin | CYM | CPC | Chy

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from calf stomach; ≥10 U/mg enzyme powder; ≥20 U/mg protein

Biochemical and Physiological Mechanisms

Chymosin is synthesized in the mucosa of the abomasum (fourth stomach) of young (unweaned) ruminants. The enzyme hydrolyzes casein to paracasein. Catalytic activity: Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser

Bioactivity

≥10 U/mg enzyme powder; ≥20 U/mg protein

Accession #

P00794

CAS

9001-98-3

Enzyme Commission Number

EC 3.4.23.4

Molecule Type

Enzyme

Storage and Shipping

Concentration

from calf stomach; ≥10 U/mg enzyme powder; ≥20 U/mg protein

Reconstitution

Reconstitute in water.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit will coagulate 10 mL of milk per min at 30 °C.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Peptidomimetics
      • Subclass Depsipeptides

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Peptidomimetics
• Subclass: Depsipeptides
• Intermediate Tree Nodes: Not available
• Direct Parent: Cyclic depsipeptides
• Alternative Parents: Alpha amino acid esters N-acyl-alpha amino acids and derivatives Macrolides and analogues Macrolactams Salicylamides Tricarboxylic acids and derivatives Benzamides Catechols Benzoyl derivatives 1-hydroxy-2-unsubstituted benzenoids 1-hydroxy-4-unsubstituted benzenoids Vinylogous acids Secondary carboxylic acid amides Lactones Carboxylic acid esters Oxacyclic compounds Carbonyl compounds Hydrocarbon derivatives Organic oxides Organonitrogen compounds Organopnictogen compounds
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Cyclic depsipeptide - Alpha-amino acid ester - N-acyl-alpha amino acid or derivatives - Macrolide - Macrolactam - Alpha-amino acid or derivatives - Salicylic acid or derivatives - Salicylamide - Benzamide - Benzoic acid or derivatives - Tricarboxylic acid or derivatives - Benzoyl - Catechol - 1-hydroxy-4-unsubstituted benzenoid - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Monocyclic benzene moiety - Benzenoid - Vinylogous acid - Carboxamide group - Carboxylic acid ester - Secondary carboxylic acid amide - Lactone - Organoheterocyclic compound - Carboxylic acid derivative - Oxacycle - Organic oxygen compound - Organic nitrogen compound - Carbonyl group - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Organooxygen compound - Organonitrogen compound - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as cyclic depsipeptides. These are natural or synthetic compounds having sequences of amino and hydroxy carboxylic acid residues (usually α-amino and α-hydroxy acids) connected in a ring. The residues are commonly but not necessarily regularly alternating.
• External Descriptors: polyphenol - macrocycle - crown compound - organic aromatic compound

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

References

1. Xiaokang Lu, Tianle Qi, Linjiang Guo, Lin Xiao, Hanbin Xu, Guobao Ning, Hui Zhao, Canpeng Li.  (2024)  A novel fluorescence sensor for milk clotting enzyme chymosin using peptide as substrate and covalent organic framework nanosheet as fluorescence quencher.  Food Science and Human Wellness,      [PMID:] [10.26599/FSHW.2023.9250042]