RFC4 Mouse mAb - Primärer Antikörper, specific to RFC4

Cat. No.: Ab125155

GRADE & PURITY Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Maus

Reactive species

Affe, Allerdings, Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

OTI1A8

Conjugation

Unconjugated

Übersicht

Mouse anti Human RFC4 Antibody, Monoclonal (OTI1A8), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

RFC4 Mouse mAb - Primärer Antikörper, specific to RFC4

Synonyme

A1 37 antibody | A1 37 kDa subunit antibody | Activator 1 37 antibody | Activator 1 37 kDa subunit antibody | Activator 1 subunit 4 antibody | Replication factor C 37 antibody | Replication factor C 37 kDa subunit antibody | Replication factor C subunit 4

Spezifikationen & Reinheit

ExactAb™, Validated, High Performance, See COA

Biochemische und physiologische Mechanismen

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA templ

Wirtsspezies

Maus

Spezifität

RFC4

Immunogen

Recombinant full length protein of human RFC4 (AA 1-363).

Positive Kontrolle

WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human pancreas, pancreas carcinoma, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY RFC4.

Konjugation

Unconjugated

Note

ExactAb™, High Performance, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - Western Blot
All lanes: RFC4 Mouse mAb (Ab125155) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 61.2 s

IHC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155)

IHC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).

IHC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).

IF/ICC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IF/ICC
RFC4 Mouse mAb (Ab125155) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.

Anwendung

• Anwendung: Dilution info
• IF/ICC:

  1/100;

• IHC:

  1/150

• WB:

  1/200-1/4000

Zugehörige Ziele (menschlich)

RFC4 Tbio Replication factor C subunit 4 (0 Activities)

Discover Target: RFC4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RFC4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.