Cartilage tissue is composed of chondrocytes and cartilage matrix. Cartilage tissue and the surrounding perichondrium constitute cartilage. According to the different fibrous components in the matrix, cartilage is classified into hyaline cartilage, elastic cartilage, and fibrocartilage. There are various staining methods for cartilage, such as toluidine blue staining, alcian blue staining, safranin O staining, etc.
The staining principle of Safranin O‑Fast Green Staining Solution for bone tissue is that basophilic cartilage binds to the basic dye safranin O and appears red, while acidophilic bone binds to the acidic dye fast green and appears green or blue. This forms a sharp contrast with the red‑stained cartilage, thereby distinguishing cartilage tissue from bone tissue. Safranin O is a cationic dye that binds to polyanions. It stains cartilage based on the binding between cationic dye and anionic groups in polysaccharides (chondroitin sulfate or keratan sulfate). The staining intensity of safranin O is approximately proportional to the concentration of anions, indirectly reflecting the content and distribution of proteoglycans in the matrix. When cartilage is injured, glycoproteins in the cartilage are released, resulting in uneven distribution of matrix components, which leads to weak or absent staining by safranin O. Quantitative analysis of safranin O‑stained cartilage matrix can be performed using image analysis software. The acidic dye fast green binds to acidophilic components in the tissue and stains them green or blue. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
B1511536
| Component
| 3×100mL
| Storage
|
B1511536A
| Fast Green Staining Solution
| 100mL
| RT
|
B1511536B
| Acid Ethanol Differentiation Solution
| 100mL
| RT
|
B1511536C
| Safranin O Staining Solution
| 100mL
| RT |
Materials Required (User-supplied):
1. 10% Formalin Fixative, Decalcifying Solution, Distilled Water, Graded Ethanol
2. Xylene or Environment-friendly Dewaxing & Clearing Agent, Neutral Balsam or Environment-friendly Mounting Medium
Protocol (For Reference Only):
1. Specimen Processing: Fix in 10% formalin, decalcify, prepare paraffin sections, dewax with xylene or dewaxing & clearing agent, and rehydrate to water.
2. Stain with Fast Green Staining Solution for 1–5 minutes.
3. Differentiate rapidly in 1% Hydrochloric Acid Ethanol for 10–15 seconds, rinse briefly with tap water.
4. Stain with Safranin O Staining Solution for 2–5 minutes.
5. Dehydrate rapidly in anhydrous ethanol four times, 3–5 seconds each time. Examine under microscope after the fourth dehydration: cartilage should appear red, and the background colorless.
6. Clear with xylene or environment-friendly dewaxing & clearing agent, mount with neutral balsam, examine under microscope, and perform image acquisition and analysis.
Staining Results:
Cartilage: Red or orange-red
Osteogenesis: Green
Connective Tissue: Red
Precautions:
1. If nuclear staining is required, iron hematoxylin staining is recommended for strong and intense staining.
2. Prolonged staining in Safranin O Staining Solution should be avoided, as it may mix with green to produce a purplish-blue color.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
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