SIGLEC H Rat mAb (AF647) - Primary antibody, specific to SIGLEC H

Cat. No.: Ab191590

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

440c

Conjugation

AF647

Overview

Rat anti Mouse SIGLEC H Antibody, Monoclonal (440c), could be used for Flow Cytometry.

Specifications

Product Name

SIGLEC H Rat mAb (AF647) - Primary antibody, specific to SIGLEC H

Synonyms

sialic acid binding Ig like lectin H antibody | SIGLEC like protein antibody | Siglech antibody | Siglec-H | 6430529G09Rik

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

SIGLEC H

Immunogen

Purified bone marrow-derived IPCs

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: SIGLEC H Rat mAb (AF647) (Ab191590)

SIGLEC H Rat mAb (AF647) (Ab191590) - Flow Cytometry
Multicolor flow cytometric analysis of SIGLEC H expression on mouse leucocytes. C57BL/6 mouse splenocytes were preincubated with CD16/CD32 Rat mAb (A412018). Cells were stained with CD45R Rat mAb (PE-Cy7) (Ab191673), CD11c Armenian Hamster mAb (FITC) (Ab178874) and either Rat IgG (AF647) (Ab190071; Left panel) or SIGLEC H Rat mAb (AF647) (Ab191590; Right panel) at 0.1 μg/test. A bivariate pseudocolor density plot showing the correlated expression of SIGLEC H expression (or Isotype control staining) was derived from CD45R+ gated events with the forward and side light-scatter characteristics of viable thymocytes.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD1C Tbio T-cell surface glycoprotein CD1c (0 Activities)

Discover Target: CD1C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)