SOX17 Mouse mAb - Primärer Antikörper, specific to SOX17

Cat. No.: Ab128771

GRADE & PURITY Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, WB

Host species

Maus

Reactive species

Allerdings, Maus, Mensch, Rat

Isotype

Mouse IgG2a

Clone Number

OTI3H5

Conjugation

Unconjugated

Übersicht

Rabbit anti Human SOX17 Antibody, Monoclonal (OTI3H5), could be used for WB, IF/ICC and so on.

Specifications

Product Name

SOX17 Mouse mAb - Primärer Antikörper, specific to SOX17

Synonyme

FLJ22252 | SOX17 | SRY (sex determining region Y)-box 17 | SRY-related HMG-box transcription factor SOX17 | transcription factor SOX-17 | VUR3 | FLJ22252 antibody | SOX17 antibody | SOX17_HUMAN antibody | SRY (sex determining region Y) box 17 antibody | S

Spezifikationen & Reinheit

ExactAb™, Validated, High Performance, See COA

Biochemische und physiologische Mechanismen

Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays

Wirtsspezies

Maus

Spezifität

SOX17

Immunogen

Recombinant protein of human SOX17 (AA 177-414)

Positive Kontrolle

WB: HeLa, HT-29, A549, MCF7 and Hep G2 cell lysates. IF/ICC: COS7cells transiently transfected with pCMV6-ENTRY SOX17.

Konjugation

Unconjugated

Note

ExactAb™, High Performance, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: SOX17 Mouse mAb (Ab128771)

SOX17 Mouse mAb (Ab128771) - Western Blot
All lanes: SOX17 Mouse mAb (Ab128771) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 44 kDa
Observed band size: 49 kDa
Exposure time: 1.9 s

IF/ICC: SOX17 Mouse mAb (Ab128771)

SOX17 Mouse mAb (Ab128771) - IF/ICC
SOX17 Mouse mAb (Ab128771) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17.

IF/ICC: SOX17 Mouse mAb (Ab128771)

SOX17 Mouse mAb (Ab128771) - IF/ICC
Immunofluorescent staining of HeLa cells using SOX17 Mouse mAb (Ab128771) .

Anwendung

• Anwendung: Dilution info
• WB:

  1/1000 - 1/5000 ;

• IF/ICC:

  1:100 ;

Zugehörige Ziele (menschlich)

SOX17 Tbio Transcription factor SOX-17 (0 Activities)

Discover Target: SOX17

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SOX17 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.