GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Suitable for Immunofluorescence(IF)?IF grade — validated for immunofluorescence with low background staining. Use to localize targets in cells/tissue by fluorescence microscopy.0.2 mg/mL
Suitable for Immunofluorescence(IF), Azide Free, Validated, 0.2 mg/mL Azide Free,Suitable for Immunofluorescence(IF),Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
The Recombinant Streptavidin Protein (APC-AF750) streptavidin tandem conjugate has excitation and emission maxima at ∼650/790 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications following excitation at 633 nm or 647 nm.
Specifications
Product Name
Recombinant Streptavidin Protein (APC-AF750), CAS No.S639027
Synonyms
SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin
Grade
Azide Free, Suitable for Immunofluorescence(IF), Validated
Specifications & Purity
Suitable for Immunofluorescence(IF), Azide Free, Validated, 0.2 mg/mL
Biochemical and Physiological Mechanisms
Streptomycin is a biotin binding protein that can bind to four types of biotin molecules. The biotin binding pocket of streptavidin facilitates interaction with biotin. It can be used to enhance protein binding and polymerization.
Bioactivity
Binding Biotin
Conjugation
Excitation (nm)
Red Laser(633nm)
Emission(nm)
790nm
Ex/Em(nm)
CAS
S639027
Molecule Type
Protein
Storage and Shipping
Shape
Solution
Concentration
0.2 mg/mL
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant Streptavidin Protein (APC-AF750) (S639027) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (APC-AF750) (S639027) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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