TCR γ/δ Mouse mAb (Biotin) - Primary antibody, specific to TCR gamma + TCR delta

Cat. No.: Ab210036

GRADE & PURITY Validated, ExactAb™

Application

Flow Cytometry

Host species

Mouse

Reactive species

Rat

Isotype

Mouse IgG1

Clone Number

V65

Conjugation

Biotin

Overview

Mouse anti Rat TCR γ/δ Antibody, Monoclonal (V65), could be used for Flow Cytometry.

Specifications

Product Name

TCR γ/δ Mouse mAb (Biotin) - Primary antibody, specific to TCR gamma + TCR delta

Synonyms

Tcrg | Trg@ | delta polypeptide | T cell receptor gamma locus | T3D | T-cell antigen receptor, gamma polypeptide | T-cell rearranging gene, gamma | T-cell receptor, gamma cluster | TCR gamma/delta | TiT3 | TRG | CD_antigen=CD3d antibody | CD3D antibody |

Specifications & Purity

ExactAb™, Validated

Host species

Mouse

Specificity

TCR gamma + TCR delta

Immunogen

Rat T Blasts

Positive Control

Flow Cytometry: Rat  splenocytes ​

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: TCR γ/δ Mouse mAb (Biotin) (Ab210036)

TCR γ/δ Mouse mAb (Biotin) (Ab210036) - Flow Cytometry
Flow cytometry analysis of TCR γ/δ expression by rat splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with TCR γ/δ Mouse mAb (Biotin) (Ab210036) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: TCR γ/δ Mouse mAb (Biotin) (Ab210036)

TCR γ/δ Mouse mAb (Biotin) (Ab210036) - Flow Cytometry
Flow cytometry analysis of TCR γ/δ expression by rat splenocytes (red) compared to an isotype control (blue). Cells were stained with TCR γ/δ Mouse mAb (Biotin) (Ab210036) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;