Type X, lyophilized powder, 30-350 units/mg protein (E1%/280) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Thermolysin is a protease that has specificity different from other proteases available for sequence investigations.
A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.
Thermolysin has been shown to have a prosequeence that acts as an intramolecular chaperone in vivo. It has also been used in a study to investigate the effects of sodium chloride on thermal stability and catalytic activity.
Thermolysin is also commonly used for the commercial synthesis of N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, the precursor for the artificial sweetener aspartame.
Specifications
Product Name
Thermolysin from Geobacillus stearothermophilus, CAS No.9073-78-3
Specifications & Purity
Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)
CAS
9073-78-3
Enzyme Commission Number
3.4.24.27(BRENDA, IUBMB)
Molecule Type
Enzyme
Storage and Shipping
Concentration
Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
1.Xiang Ming, Lin Xiangdong, Chen Haiying. (2025) Luteolin Inhibits Ferroptosis of HUVEC by Regulating the Sirt1/Nrf2 Pathway. CELL BIOCHEMISTRY AND BIOPHYSICS, [PMID:40721691][10.1007/s12013-025-01831-1]
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