GRADE & PURITYMycoplasma free?Mycoplasma-free — tested negative for mycoplasma contamination. Use in cell culture to prevent silent infections that skew experiments.
Mycoplasma free Mycoplasma free for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research.
Specifications
Product Name
THP-1 Whole Cell Lysate
Grade
Mycoplasma free
Specifications & Purity
Mycoplasma free
Molecule Type
Cell or Tissue Extract
Storage and Shipping
Shape
Lyophilized
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Images
THP-1 Whole Cell Lysate (cl185703) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 53.8 s
THP-1 Whole Cell Lysate (cl185703) - Western Blot All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 31 kDa Exposure time: 20.0 s
THP-1 Whole Cell Lysate (cl185703) - Western Blot All lanes: Recombinant Hsp90 Antibody (Ab108929) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane SSecondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time: 53.5 s
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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