Transferrin Receptor/CD71 Mouse mAb - Primary antibody, specific to Transferrin Receptor/CD71, Mouse IgG1

Cat. No.: Ab132254

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Flow Cytometry, IF/ICC, IHC

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

OKT-9

Conjugation

Unconjugated

Overview

Mouse anti Human Transferrin Receptor/CD71 Antibody, Monoclonal (OKT-9), could be used for IHC, ICC, IF, Flow and so on.
Application:
Flow: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
IF/ICC: Use at an assay dependent concentration.
Protein Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Specifications

Product Name

Transferrin Receptor/CD71 Mouse mAb - Primary antibody, specific to Transferrin Receptor/CD71, Mouse IgG1

Synonyms

TFR | TRFR | IMD46 | CD71 antigen | CD71 | CD71TRFR | p90 | T9 | TfR (Transferrin R) | TfR (TransferrinR) | TfR | TFR1 | TFRC | TR | transferrin receptor (p90, CD71) | transferrin receptor protein 1 | CD 71 antibody | CD71 antibody | CD71 antigen antibody

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Mouse

Specificity

Transferrin Receptor/CD71

Immunogen

Human acute lymphoblastic leukemia cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated Jurkat cells (24h) labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of MOLT-4 cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated MOLT-4 cells (24h) labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 2.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 2.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IHC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• IF/ICC:

  Use at an assay dependent concentration.;

Associated Targets(Human)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action