TRAP alpha/TRAPA Mouse mAb - Primärer Antikörper, specific to SSR1

Cat. No.: Ab132305

GRADE & PURITY Validated, KO Validation, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Maus

Reactive species

Affe, Allerdings, Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

OTI3E5

Conjugation

Unconjugated

Übersicht

Mouse anti Human TRAP alpha/TRAPA Antibody, Monoclonal (OTI3E5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

TRAP alpha/TRAPA Mouse mAb - Primärer Antikörper, specific to SSR1

Synonyme

DKFZp781N23103 antibody | FLJ14232 antibody | FLJ22100 antibody | FLJ23034 antibody | FLJ78242 antibody | FLJ93042 antibody | PGP35 antibody | Signal sequence receptor alpha antibody | Signal sequence receptor alpha subunit antibody | Signal sequence rece

Spezifikationen & Reinheit

KO Validation, ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation pro

Wirtsspezies

Maus

Spezifität

SSR1

Immunogen

Recombinant full length protein of human TRAP alpha/TRAPA.

Positive Kontrolle

WB: Hep G2, HeLa, A549, Jurkat and A431 cell lysates. IHC: Human ovary adenocarcinoma, pancreas, endometrium, endometrium adenocarcinoma, prostate tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY SSR1. Flow Cytometry: HEK293T cells tra

Konjugation

Unconjugated

Note

ExactAb™, KO Validation, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - Western Blot
All lanes: TRAP alpha/TRAPA Mouse mAb (Ab132305) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 34 kDa
Exposure time: 120.0 s

Western Blot: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - Western Blot
Equivalent amounts of cell lysates (10 ug perlane) of wild-type HeLa cells and SSR1-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with TRAP alpha/TRAPA Mouse mAb (Ab132305)  (1/2000). Then the blotted membrane was stripped and reprobed with anti- HSP90 antibody as a loading control.

IHC: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)

IHC: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)

IHC: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)

IHC: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)

IHC: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)

IF/ICC: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - IF/ICC
TRAP alpha/TRAPA Mouse mAb (Ab132305) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSR1.

Flow Cytometry​: TRAP alpha/TRAPA Mouse mAb (Ab132305)

TRAP alpha/TRAPA Mouse mAb (Ab132305) - Flow Cytometry​
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by TRAP alpha/TRAPA Mouse mAb (Ab132305) and then analyzed by flow cytometry.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/150

Zugehörige Ziele (menschlich)

SSR1 Tbio Translocon-associated protein subunit alpha (0 Activities)

Discover Target: SSR1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TRAP alpha/TRAPA Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.