TRIM33 Maus-mAb - Primärer Antikörper, specific to TRIM33, Mouse IgG2a

Cat. No.: Ab132507

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2a

Clone Number

3H7C8B9

Conjugation

Unconjugated

Übersicht

Maus-Antihuman-Antikörper gegen TRIM33, monoklonal (3H7C8B9), kann für WB und so weiter verwendet werden.

Specifications

Product Name

TRIM33 Maus-mAb - Primärer Antikörper, specific to TRIM33, Mouse IgG2a

Synonyme

ECTO | PTC7 | RFG7 | TF1G | TIF1G | TIFGAMMA | TIF1GAMMA | 8030451N04Rik-Antikörper | AI413936-Antikörper | cb1085-Antikörper | DKFZp586K1123-Antikörper | E3-Ubiquitin-Protein-Ligase TRIM33-Antikörper | EC 6.3.2.--Antikörper | Ectodermin-Antikörper | Ecto

Spezifikationen & Reinheit

ExactAb™, validiert, 0,5 mg/mL

Biochemische und physiologische Mechanismen

Wirkt als E3-Ubiquitin-Protein-Ligase. Fördert die Ubiquitinierung von SMAD4, den Ausschluss aus dem Kern und den Abbau über den Ubiquitin-Proteasom-Weg. Laut PubMed:16751102 führt es nicht zu einer Verringerung der Menge an endogenem SMAD4. Kann als Tran

Wirtsspezies

Maus

Spezifität

TRIM33

Immunogen

Recombinant protein of human TIF1 gamma / TRIM33

Positive Kontrolle

WB: MCF7 and HeLa lysates.

Konjugation

Unconjugated

Note

ExactAb™, Validated

Isotyp

Mouse IgG2a

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Flüssigkeit

Storage

Bei -20°C lagern, wiederholtes Einfrieren und Auftauen vermeiden

Verschickt in

Eistruhe + Eispads

Stabilität und Lagerung

Kurzfristig bei 4°C lagern (1-2 Wochen). Langfristig bei -20°C lagern (12 Monate). Es wird empfohlen, nach Erhalt zu aliquotieren. Einfrier-/Auftauzyklus vermeiden.

Bilder

Western Blot: TRIM33 Mouse mAb (Ab132507)

TRIM33 Mouse mAb (Ab132507) - Western Blot
All lanes: TRIM33 Mouse mAb (Ab132507) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 123 kDa
Observed band size: 154 kDa
Exposure time: 53.2 s

Western Blot: TRIM33 Mouse mAb (Ab132507)

TRIM33 Mouse mAb (Ab132507) - Western Blot
All lanes: TRIM33 Mouse mAb (Ab132507) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 123 kDa
Observed band size: 149 kDa
Exposure time: 100.0 s

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

Zugehörige Ziele (menschlich)

TRIM33 Tchem E3 ubiquitin-protein ligase TRIM33 (0 Activities)

Discover Target: TRIM33

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TRIM33 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.