GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.KO Validation?Knockout-validated — antibody specificity confirmed using knockout cells/tissue. Use when you need strong evidence the antibody binds only its target.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
KO Validation, ExactAb™, Validated, See COA ExactAb™,KO Validation,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Mouse anti Human TTLL12 Antibody, Monoclonal (OTI4B2), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.
Specifications
Product Name
TTLL12 Mouse mAb - Primärer Antikörper, specific to TTLL12
Recombinant full length protein of human TTLL12 produced in HEK293T.
Positive Kontrolle
WB: 293T, HT-29 and Jurkat cell lysates. IHC: Human breast, adenocarcinoma of breast, colon, adenocarcinoma of colon, kidney, liver, carcinoma of liver, ovary, adenocarcinoma of ovary, pancreas, thyroid, endometrium, adenocarcinoma of endometrium, prostat
Konjugation
Unconjugated
Note
ExactAb™, KO Validation, Validated
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein G purified
Konzentration
See COA
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
TTLL12 Mouse mAb (Ab132973) - Western Blot All lanes: TTLL12 Mouse mAb (Ab132973) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution Predicted band size: 74 kDa Observed band size: 75 kDa Exposure time: 64.8 s
TTLL12 Mouse mAb (Ab132973) - Western Blot Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT) and TTLL12-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with TTLL12 Mouse mAb (Ab132973) (1/500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control.
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IHC Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)
TTLL12 Mouse mAb (Ab132973) - IF/ICC Immunofluorescent staining of Hep G2 cells using TTLL12 Mouse mAb (Ab132973).
TTLL12 Mouse mAb (Ab132973) - Flow HEK293T cells transfected with either pCMV6-ENTRY TTLL12 (Red) or empty vector control plasmid (Blue) were immunostained with TTLL12 Mouse mAb (Ab132973) (dilution 1/1000), and then analyzed by flow cytometry.
TTLL12 Mouse mAb (Ab132973) - Flow Flow cytometric analysis of HeLa cells, using TTLL12 Mouse mAb (Ab132973) (Red) compared to a nonspecific negative control antibody (Blue).
TTLL12 Mouse mAb (Ab132973) - Flow Flow cytometric analysis of Jurkat cells, using TTLL12 Mouse mAb (Ab132973) (Red) compared to a nonspecific negative control antibody (Blue).
Anwendung
Anwendung
Dilution info
WB
1/2000
Flow Cytometry
1/100
IF/ICC
1/100
IHC
1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Bewertungen
Kundenbewertungen
Häufig gestellte Fragen
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does KO Validation mean?
KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TTLL12 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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