GRADE & PURITYEnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.0.5 μg/μl in PBS containing 0.01% sodium azide
EnzymoPure™, 0.5 μg/μl in PBS containing 0.01% sodium azide EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
Specifications
Product Name
Ubiquitin E1 Enzyme (UBE1), human recombinant, CAS No.U388907
Grade
EnzymoPure™
Specifications & Purity
EnzymoPure™, 0.5 μg/μl in PBS containing 0.01% sodium azide
CAS
U388907
Molecule Type
Enzyme
Storage and Shipping
Concentration
0.5 μg/μl in PBS containing 0.01% sodium azide
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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