GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)Protein concentration: See COA; ≥500 U/mg protein; expressed in E.coli
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),Protein concentration: See COA; ≥500 U/mg protein; expressed in E.coli ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Uridine-5′-diphosphoglucose pyrophosphorylase (UGP) (EC: 2.7.7.9), derived from Leishmania major. It catalyzes the formation of UDP-glucose from UTP and glucose 1-phosphate.This enzyme exhibits high substrate specificity: it cannot utilize other NTPs (e.g., dTTP, CTP, ATP, GTP) or other sugar-1-phosphates (e.g., GlcNAc-1P, Gal-1P, Man-1P) as substrates.
Reaction:
Protocol for Use
Add substrates to the reaction mixture, supplemented with 5 mM MgCl₂. Adjust pH to 7.5–8.5 using an appropriate buffer (e.g., Tris-HCl), then add an appropriate amount of UGP (adjust according to reaction requirements).
Mix thoroughly and incubate at 37°C for 30–60 minutes.
Note: For scaled-up reactions, adjust incubation time or enzyme amount based on actual conditions.
Precautions
For your safety and health, please wear lab coats and disposable gloves when handling. This product is for research use only!
Specifications
Product Name
Uridine-5′-diphosphoglucose pyrophosphorylase (UGP), CAS No.9026-22-6
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), Protein concentration: See COA; ≥500 U/mg protein; expressed in E.coli
Protein concentration: See COA; ≥500 U/mg protein; expressed in E.coli
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Unit definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol UDP-α-D-glucose from α-D-glucose 1-phosphate and UTP per minute at 37 °C.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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