Urokinase from human urine, CAS No.9039-53-6

CAS: 9039-53-6 Cat. No.: U108373 EC Number: 232-917-9

GRADE & PURITY EnzymoPure™, ≥85%, from human urine; Potency ≥50000 IU/mg; ≥120000 IU/mg protein

Accession #

P00749

Endotoxin Concentration

≤1.0 EU/10000 IU

Bioactivity

≥50000 IU/mg enzyme powder; ≥120000 IU/mg protein

Overview

  Urokinase is a highly specific alkaline protease isolated from the urine of healthy men. The molecular weight is mainly composed of 33000 and 54000 whose isoelectric point is PH 8 ~ 9. Urokinase powder made by the steps of extraction process of urine after silica gel adsorption, ammonium sulfate precipitation, filtration, Chromatography purification, sterilization, freeze drying , soluble in water, alkaline protease is a highly specific. 

  Urokinase is a kind of enzyme thrombolytic agent, which can activate plasminogen into plasmin, which can hydrolyze fibrin to form thrombus. It is mainly used in the treatment of acute myocardial infarction, acute cerebral thrombosis and cerebral embolism, peripheral arterial and venous thrombosis, central retinal artery and vein thrombosis and other fresh thrombotic occlusive diseases. For formation, peripheral vascular embolism, central retinal vein embolism, acute myocardial infarction and other fresh thromboembolic disease in the treatment of cerebral thrombosis, and renal transplantation, plastic surgery and thrombosis.

Specifications

Product Name

Urokinase from human urine, CAS No.9039-53-6

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥85%, from human urine; Potency ≥50000 IU/mg; ≥120000 IU/mg protein

Biochemical and Physiological Mechanisms

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. Catalytic activity: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. Activity regulation: Inhibited by SERPINA5. Inhibited by SERPINE1. Post-translational:

Bioactivity

≥50000 IU/mg enzyme powder; ≥120000 IU/mg protein

Endotoxin Concentration

≤1.0 EU/10000 IU

Accession #

P00749

Predicted molecular weight

54 kDa

CAS

9039-53-6

Enzyme Commission Number

EC 3.4.21.73

Molecule Type

Enzyme

Storage and Shipping

Concentration

from human urine; Potency ≥50000 IU/mg; ≥120000 IU/mg protein

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzimidazoles

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzimidazoles
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Benzimidazoles
• Alternative Parents: Benzenoids Thiazoles Imidazoles Heteroaromatic compounds Propargyl-type 1,3-dipolar organic compounds Carboximidic acids and derivatives Azacyclic compounds Organopnictogen compounds Organooxygen compounds Organonitrogen compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Benzimidazole - Benzenoid - Azole - Imidazole - Thiazole - Heteroaromatic compound - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Azacycle - Carboximidic acid derivative - Organic oxygen compound - Organic nitrogen compound - Organooxygen compound - Organonitrogen compound - Hydrocarbon derivative - Organopnictogen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as benzimidazoles. These are organic compounds containing a benzene ring fused to an imidazole ring (five member ring containing a nitrogen atom, 4 carbon atoms, and two double bonds).
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 (345557 Activities)

Discover Target: TDP1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GMNN Tbio Geminin (128009 Activities)

Discover Target: GMNN

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

Mus musculus (284745 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Luciferin 4-monooxygenase (66902 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Rorc Nuclear receptor ROR-gamma (89407 Activities)

Discover Target: Rorc

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

References

1. Zhechang Chen, Caijie Yuan, Yaxin Ye, Bitao Lu, Enling Hu, Fei Lu, Kun Yu, Ruiqi Xie, Guangqian Lan.  (2023)  Dual-targeting fucoidan-based microvesicle for arterial thrombolysis and re-occlusion inhibition.  CARBOHYDRATE POLYMERS,      [PMID:38220339] [10.1016/j.carbpol.2023.121703]

2. Song Jianwen, Kang Xiaoying, Wang Lu, Ding Dan, Kong Deling, Li Wen, Qi Ji.  (2023)  Near-infrared-II photoacoustic imaging and photo-triggered synergistic treatment of thrombosis via fibrin-specific homopolymer nanoparticles.  Nature Communications,  14  (1): (1-19).  [PMID:37898604] [10.1038/s41467-023-42691-8]

3. Weiwei Zhang, Yuguo Deng, Jinhao Zhao, Tao Zhang, Xiang Zhang, Wenping Song, Lin Wang, Tianlong Li.  (2023)  Amoeba-Inspired Magnetic Venom Microrobots.  Small,  19  (23): (2207360).  [PMID:36869412] [10.1002/smll.202207360]

4. Mei Jian feng, Cai Shao fen, Yi Yu, Wang Xu dong, Ying Guo qing.  (2023)  Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects.  Brazilian Journal of Pharmaceutical Sciences,      [PMID:] [10.1590/s2175-97902022e201004]

5. Songzhi Xie, Shang Li, Wenxiong Cao, Chuanfei Mo, Zhanlin Zhang, Kun Huang, Xiaohong Li.  (2022)  Bacteria-Templated and Dual Enzyme-Powered Microcapsule Motors To Promote Thrombus Penetration and Thrombolytic Efficacy.  ACS Applied Materials & Interfaces,      [PMID:35948498] [10.1021/acsami.2c11213]

6. Dongya Wang, Qianqian Zhao, Jingcan Qin, Yuanyuan Guo, Chuan Zhang, Yuehua Li.  (2022)  Urokinase loaded black phosphorus nanosheets for sequential thrombolysis and reactive oxygen species scavenging in ischemic stroke treatment.  Biomaterials Science,  10  (16): (4656-4666).  [PMID:35801526] [10.1039/D2BM00746K]

7. Songzhi Xie, Chuanfei Mo, Wenxiong Cao, Shuang Xie, Shang Li, Zhanlin Zhang, Xiaohong Li.  (2022)  Bacteria-propelled microtubular motors for efficient penetration and targeting delivery of thrombolytic agents.  Acta Biomaterialia,      [PMID:35158079] [10.1016/j.actbio.2022.02.008]

8. Yina Su, Linjie Huang, Guizhen Xu, Simin Chen, Jiaqiong Wu, Siyu Wang, Yichao Zhang, Xiahui Lin.  (2025)  NIR-Propelled Thermosensitive Bowl-Shaped Nanomotors with High Penetration and Targeting for Photoacoustic Imaging Guided Thrombolysis Therapy.  Advanced Healthcare Materials,      [PMID:40125829] [10.1002/adhm.202404960]

9. Chong Chen, Ran Xiao, Shuang Du, Jingni Xu, Pengjie Wang, Yang Song, Xiaobing Wang, Han Chen, Tianjiao Niu, Weibo Zhang.  (2026)  Effect of high-pressure jet treatment on the plasmin system in milk.  Applied Food Research,  6  (1): (101796).  [PMID:] [10.1016/j.afres.2026.101796]