VAMP8/EDB Mouse mAb - Primärer Antikörper, specific to VAMP8, Mouse IgG1

Cat. No.: Ab133867

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, 0.5 mg/mL

Application

IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

1414CT354.12.23.93

Conjugation

Unconjugated

Übersicht

Mouse anti Human VAMP8/EDB Antibody, Monoclonal (1414CT354.12.23.93), could be used for WB, IHC and so on.
Application
· WB: 1/1000-1/2000
· IHC: 1/100-1/500
Protein Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Specifications

Product Name

VAMP8/EDB Mouse mAb - Primärer Antikörper, specific to VAMP8, Mouse IgG1

Synonyme

EDB | Endobrevin | VAMP-8 | EDB antibody | Endobrevin antibody | VAMP 8 antibody | VAMP-8 antibody | VAMP8 antibody | VAMP8_HUMAN antibody | Vesicle associated membrane protein 8 antibody | Vesicle-associated membrane protein 8 antibody | EDB | VAMP-8

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL

Wirtsspezies

Maus

Spezifität

VAMP8

Immunogen

A KLH conjugated synthetic peptide from the N-terminal region of human VAMP8 (AA 2-24).

Positive Kontrolle

WB: THP-1 and K562 cell lysates. IHC: Human brain tissue.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, High Performance, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: VAMP8/EDB Mouse mAb (Ab133867)

VAMP8/EDB Mouse mAb (Ab133867) - Western Blot
All lanes: VAMP8/EDB Mouse mAb (Ab133867) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 15 kDa
Exposure time: 26.4 s

IHC: VAMP8/EDB Mouse mAb (Ab133867)

VAMP8/EDB Mouse mAb (Ab133867) - IHC
Immunohistochemical analysis of paraffin-embedded H. brain section using VAMP8/EDB Mouse mAb (Ab133867) (N-term). VAMP8/EDB Mouse mAb (Ab133867) was diluted at 1/25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anwendung

• Anwendung: Dilution info
• IHC:

  1/100-1/500;

• WB:

  1/1000 - 1/2000

Zugehörige Ziele (menschlich)

VAMP8 Tbio Vesicle-associated membrane protein 8 (0 Activities)

Discover Target: VAMP8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (VAMP8/EDB Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.