Vinculin Mouse mAb (AF647) - Primary antibody, specific to Vinculin

Cat. No.: Ab219200
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
AB01/1F7
Conjugation
AF647
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab219200-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$47.90
50μl
Ab219200-50μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$143.90
100μl
Ab219200-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$227.90
1ml
Ab219200-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Vinculin Antibody, Monoclonal (AB01/1F7), could be used for Flow Cytometry.

Specifications

Product Name
Vinculin Mouse mAb (AF647) - Primary antibody, specific to Vinculin
Synonyms
CMD1W antibody | CMH15 antibody | Epididymis luminal protein 114 antibody | HEL114 antibody | Metavinculin antibody | MV antibody | MVCL antibody | OTTHUMP00000019861 antibody | OTTHUMP00000019862 antibody | VCL antibody | VINC antibody | VINC_HUMAN antib
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Host species
Mouse
Specificity
Vinculin
Immunogen
Recombinant full length protein corresponding to Human Vinculin
Positive Control
Flow Cytometry: HEK293 cells
Conjugation
AF647
Excitation (nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images

Vinculin Mouse mAb (AF647) (Ab219200) - Flow Cytometry
Flow cytometry analysis of Vinculin expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Vinculin Mouse mAb (AF647) (Ab219200) (red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

1:50-1:500

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
VCL Tbio Vinculin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0826402Certificate of AnalysisJun 20, 2026 Ab219200
ZJ25F0826403Certificate of AnalysisJun 20, 2026 Ab219200
ZJ25F0826404Certificate of AnalysisJun 20, 2026 Ab219200
Solution Calculators
Reviews

Customer Reviews

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.