GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Native?Native grade — protein/biomolecule in its natural (non-recombinant, non-denatured) form. Use when native structure and activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.from Porcine Heart; ≥90 U/mg enzyme powder
Bioactive,ActiBioPure™,Native,High Performance,EnzymoPure™,from Porcine Heart; ≥90 U/mg enzyme powder ActiBioPure™,Bioactive,High Performance,Native,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Alanine Aminotransferase is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.
Alanine Aminotransferase mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate. Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
Specifications
Product Name
Alanine Aminotransferase (ALT/GPT), CAS No.9000-86-6
ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™
Specifications & Purity
Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Porcine Heart; ≥90 U/mg enzyme powder
Biochemical and Physiological Mechanisms
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Post-tran
Bioactivity
≥90 U/mg enzyme powder
N-terminal Sequence
One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
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