alpha Tubulin Mouse mAb (AF488) - Primary antibody, specific to alpha tubulin

Cat. No.: Ab219162
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG2b
Clone Number
AB01/5A12
Conjugation
AF488
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab219162-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$47.90
50μl
Ab219162-50μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$143.90
100μl
Ab219162-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$227.90
1ml
Ab219162-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,399.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human alpha tubulin Antibody, Monoclonal (AB01/5A12), could be used for Flow Cytometry.

Specifications

Product Name
alpha Tubulin Mouse mAb (AF488) - Primary antibody, specific to alpha tubulin
Synonyms
Alpha tubulin ubiquitous antibody | Alpha-tubulin ubiquitous antibody | K alpha 1 antibody | TBA1B_HUMAN antibody | TUBA1B antibody | Tubulin alpha 1B antibody | Tubulin alpha 1B chain antibody | Tubulin alpha ubiquitous antibody | Tubulin alpha ubiquitou
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Host species
Mouse
Specificity
alpha tubulin
Immunogen
Recombinant human alpha tubulin
Positive Control
Flow Cytometry: Hela cells
Conjugation
AF488
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images

alpha Tubulin Mouse mAb (AF488) (Ab219162) - Flow Cytometry
Flow cytometry analysis of alpha Tubulin expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with alpha Tubulin Mouse mAb (AF488) (Ab219162) (red) at 1/300 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

1:50-1:500

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
TUBA1B Tchem Tubulin alpha-1B chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0826318Certificate of AnalysisJun 20, 2026 Ab219162
ZJ25F0826319Certificate of AnalysisJun 20, 2026 Ab219162
ZJ25F0826320Certificate of AnalysisJun 20, 2026 Ab219162
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