Determine the necessary mass, volume, or concentration for preparing a solution.
Anti-GFP Nanobody Magnetic Beads (G1373495) - IP
All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/5000 dilution
Lane1: HEK-293T transfected with an empty vector, whole cell lysate
Lane2: HEK-293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate
Lane3: Anti-GFP Nanobody Magnetic Beads (G1373495) IP in HEK-293T transfected with an empty vector, whole cell lysate
Lane4: Anti-GFP Nanobody Magnetic Beads (G1373495) IP in HEK-293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 27, 29 kDa Exposure time: 30.0 s

