Cholesterol Oxidase from microorganism, CAS No.9028-76-6

CAS: 9028-76-6 Cat. No.: C139686 EC Number: 232-842-1

GRADE & PURITY EnzymoPure™, ＞10U/mg

Overview

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites. Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 °C. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage

Specifications

Product Name

Cholesterol Oxidase from microorganism, CAS No.9028-76-6

Synonyms

Oxidase cholesterol | Cholesterol: oxygen oxidoreductase | Cholesterol Oxidase microbial

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ＞10U/mg

Biochemical and Physiological Mechanisms

胆固醇氧化酶及胆固醇脂酶一起用于血脂类项目如高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）以及总胆固醇（CHO）等。

CAS

9028-76-6

Enzyme Commission Number

1.1.3.6

Molecule Type

Enzyme

Storage and Shipping

Concentration

＞10U/mg

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization

References

1. Chu Fenghong, Feng Yuan, Hu Anduo, Dai Caihong, Qi Yue, Bian Zhenglan.  (2023)  Cholesterol biosensing based on hydrogel optical fiber immobilization with cholesterol oxidase.  OPTIK,      [PMID:] [10.1016/j.ijleo.2023.170995]

2. Ke Yang, Guoning Chen, Lu Wang, Miao Guo, Jiameng Xu, Yirong Ma, Zhimin Luo, Aiguo Zeng, Qiang Fu.  (2023)  Zeolitic-imidazolate framework (ZIF-8)-based immobilized multi-enzymes integrated with a colorimetric sensor for cholesterol assay.  NEW JOURNAL OF CHEMISTRY,  47  (8): (4103-4112).  [PMID:] [10.1039/D2NJ05747F]

3. Ying Li, Shujuan Li, Min Bao, Linqun Zhang, Carlo Carraro, Roya Maboudian, Anran Liu, Wei Wei, Yuanjian Zhang, Songqin Liu.  (2021)  Pd Nanoclusters Confined in ZIF-8 Matrixes for Fluorescent Detection of Glucose and Cholesterol.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.1c01712]

4. Yanlei He, Ning Li, Weikang Li, Xianxi Zhang, Xiao Zhang, Zhenxue Liu, Qingyun Liu.  (2020)  5,10,15,20-tetrakis (4-carboxylphenyl) porphyrin functionalized NiCo2S4 yolk-shell nanospheres: Excellent peroxidase-like activity, catalytic mechanism and fast cascade colorimetric biosensor for cholesterol.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2020.128850]

5. Wang Nan, Sun Jianchao, Chen Lijian, Fan Hai, Ai Shiyun.  (2015)  A Cu2(OH)3Cl-CeO2 nanocomposite with peroxidase-like activity, and its application to the determination of hydrogen peroxide, glucose and cholesterol.  MICROCHIMICA ACTA,  182  (9): (1733-1738).  [PMID:] [10.1007/s00604-015-1506-8]

6. Chaochao Shen, Xin Xi, Dongqing Wu, Xiaojun Guo, Yuezeng Su, Ruili Liu.  (2024)  Active-matrix extended-gate field-effect transistor array for simultaneous detection of multiple metabolites.  BIOSENSORS & BIOELECTRONICS,      [PMID:39305822] [10.1016/j.bios.2024.116787]

7. Ziyi Tong, Shengyan Hou, Zhenkun Zhang, Zhen Liu, Yifei Zhang.  (2024)  Biochemical approaches for decoding the information stored with metabolites.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136618]

8. Shuangqian Yan, Panpan Xue, Ying Sun, Tingjie Bai, Sijie Shao, Xuemei Zeng.  (2024)  Cupric Doping Hollow Prussian Blue Nanoplatform for Enhanced Cholesterol Depletion: a Promising Strategy for Breast Cancer Therapy and Metastasis Inhibition.  Advanced Science,      [PMID:39606805] [10.1002/advs.202409967]

9. Pang Tingyuan, Liu Xiaorui, Li Jia, Song Qi, Li Junren, Han Ling, Shu Wenying, Jian Xiaoshun, Zhang Meimei.  (2024)  Highly sensitive and accurate detection of cholesterol based on a single red upconversion biosensor.  RSC Advances,  14  (11): (7858-7866).  [PMID:38449817] [10.1039/D3RA07354H]

10. Guo Qi, Wang Du, Ma Fei, Fang Mengxue, Zhang Liangxiao, Li Peiwu, Yu Li.  (2024)  MOF-derived nanozyme CuOx@C and its application for cascade colorimetric detection of phytosterols.  MICROCHIMICA ACTA,  191  (6): (1-14).  [PMID:38717599] [10.1007/s00604-024-06389-y]

11. Yuanyuan Zhang, Lexian wu, Jing Yang, Guoming Li, Keqin Deng, Haowen Huang.  (2024)  Platinum Glutamate Acid Complex as a Peroxidase Mimic: High Activity, Controllable Chemical Modification, and Application in Biosensor.  Analytical Methods,      [PMID:38284245] [10.1039/D3AY02121A]

12. Meiling Chen, Yang Yang, Qinhua Chen, Lina Tang, Junlin Liu, Yujie Sun, Qiming Liu, Yulin Zhang, Guo-jun Zhang, Shaowei Chen.  (2024)  Pt,P-codoped carbon nitride nanoenzymes for fluorescence and colorimetric dual-mode detection of cholesterol.  ANALYTICA CHIMICA ACTA,      [PMID:38438235] [10.1016/j.aca.2024.342351]

13. Xiangling Li, Shantao Zheng, Mengyi He, Xinshuo Huang, Cheng Yang, Jingshan Mo, Jingbo Yang, Chengduan Yang, Huijiuan Chen, Xi Xie.  (2025)  Self-calibrating multiplexed microneedle electrode array for continuous mapping of subcutaneous multi-analytes in diabetes.  The Innovation,      [PMID:39991476] [10.1016/j.xinn.2024.100781]

14. Xinying Ma, Zhe Zhang, Yanyan Zheng, Jiyang Liu.  (2024)  Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol.  Biosensors-Basel,  14  (8): (403).  [PMID:39194632] [10.3390/bios14080403]

15. Yanmei Lu, Jiayue Liu, Yufang Hu, Wenjun Dong, Caizhao Cheng, Zhihe Qing, Shun Zhang.  (2024)  Visual and quantitative determination of KAT Tip60 activity in circulating tumor cells using a smartphone.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.135827]

16. Xuemei Zeng, Shumin Tang, Guosheng Hu, Yonghong Pang, Sihuang Lin, Shijie Shangguan, Shuangqian Yan, Xinglin Ruan, Liling Zheng.  (2025)  Reprogramming Macrophage Function via Cholesterol Modulation and Redox Signaling Using a Multifunctional Nanoplatform for Postoperative Breast Cancer Management.  ACS Nano,      [PMID:40905820] [10.1021/acsnano.5c08767]

17. Panpan Xue, Tingjie Bai, Huilan Zhuang, Angelo H. All, Shuangqian Yan, Xuemei Zeng.  (2025)  Cholesterol Metabolism Regulated Nanoliposome Ameliorates Chemo/Photothermal Therapy Reversing CD8+ T Cell Exhaustion.  Exploration,      [PMID:] [10.1002/EXP.20240123]

18. Hongrui Zhang, Ya-nan Zhang, Like Li, Xuegang Li, Yong Zhao.  (2025)  An Enzyme-Catalyzed Optical Fiber Optofluidic Laser Sensor for Cholesterol Detection Using Rhodamine B Hydrazide.  ANALYTICAL CHEMISTRY,      [PMID:39982367] [10.1021/acs.analchem.4c06462]

19. Kai Yan, Chonglun Wang, Ye Zhang, Yunfei Li, Ying Jin, Jingjing Jiang, Shengda Liu, Jiayin Zhan, Wensheng Yu, Xiangting Dong, Guixia Liu.  (2025)  Protein-stabilized gold nanoclusters for colorimetric/fluorescence dual-mode detection of organophosphorus pesticides.  FOOD CHEMISTRY,      [PMID:41259851] [10.1016/j.foodchem.2025.147115]

20. Chen Hu, Niu Feng, Nana Gao, Nan Liu, Shuailong Zhang, Aiqin Luo, Yiping Chen, Jiangjiang Zhang.  (2026)  Surface Passivation and Aggregation-Enhanced Emission of Luminescent Copper Nanoparticles for Advanced Biochemical Sensors.  ANALYTICAL CHEMISTRY,      [PMID:] [10.1021/acs.analchem.5c06608]