GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Suitable for electrophoresis?Electrophoresis grade — low ionic/UV impurities for clean gel runs. Use in agarose/PAGE electrophoresis where impurities distort bands.Ready-to-use?Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors.for PAGE?PAGE grade — purity suited to polyacrylamide gel electrophoresis. Use for protein/nucleic-acid PAGE where reagent purity affects resolution.for Western blot?Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low.
BioReagent, ready-to-use, for PAGE, for western blot, suitable for electrophoresis BioReagent,for PAGE,for Western blot,Ready-to-use,Suitable for electrophoresis for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
This product is a newly developed rapid, highly sensitive, low-background, non-toxic, and environmentally friendly Coomassie Blue staining solution from Aladdin. It is suitable for rapid protein staining in SDS-PAGE and non-denaturing gels. Protein bands at the nanogram level can be visualized after just 15 minutes of staining, with extremely low background. Further background reduction can be achieved by rinsing with pure water, eliminating the need for toxic destaining solutions.
Key Features
Rapid and Sensitive: Detect protein bands as low as 10 ng with just 15 minutes of staining. Sensitivity can be further improved by extending staining time.
Low Background: Clear protein bands are visible immediately after staining. Rinsing with pure water further reduces background and improves signal-to-noise ratio.
Environmentally Friendly: Non-toxic formula with no irritating odor, ensuring user safety.
Protocol
After electrophoresis, rinse the gel in pure water for a few minutes to remove impurities and reduce background.
Discard the water and add sufficient staining solution to cover the gel. Stain on a horizontal shaker at room temperature for 10–15 minutes. For low-abundance proteins, extend the staining time as needed. After staining, discard the solution and rinse with pure water to remove residual dye.
For further background reduction, replace the pure water every 20 minutes and repeat 4–5 times. Alternatively, incubating the stained gel in pure water overnight can achieve even lower background and clearer bands.
Precautions
This reagent contains volatile compounds. Ensure tight sealing to avoid loss of efficacy.
If protein bands are faint after staining, it may indicate low protein content. Extend staining or rinsing time. Include a positive control during sample loading to validate staining performance.
SDS can interfere with Coomassie Blue-protein binding. Pre-rinse the gel with warm pure water to remove residual SDS for improved staining results.
Wear a lab coat and disposable gloves for safety.
For research use only.
Specifications
Specifications & Purity
BioReagent, ready-to-use, for PAGE, for western blot, suitable for electrophoresis
Stability And Storage
Store at 2-8℃ long term (12 months). Store in the dark.
Storage
Store at 2-8°C, Protected from light
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, for PAGE, for Western blot, Ready-to-use, Suitable for electrophoresis
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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