Cystathionine-β-Lyase (CBL), CAS No.9055-05-4

CAS: 9055-05-4 Cat. No.: C774055

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), ≥90 U/mg protein

Accession #

F5A553

Expression system

E. coli

Bioactivity

≥90 U/mg protein; Unit definition: One unit of activity is defined as the production of 1μmol of L-homocysteine per min at 37℃ at pH8.0.

Overview

Cystathionine-β-lyase (CBL, EC 4.4.1.8) represents a pivotal member of the lyase family, demonstrating exceptional substrate specificity. The enzyme catalyzes the β-cleavage of L-cystathionine with high precision, generating homocysteine (Hcy) through the sequential elimination of pyruvate and ammonia moieties.

Reaction

Synergistic Catalytic System with CBS‌
1.CBL forms an efficient catalytic tandem with cystathionine-β-synthase (CBS, EC 4.2.1.22, Aladdin No. C774059) in the cyclic Hcy detection mechanism:

‌2.Condensation Phase‌: CBS initiates the reaction by condensing Hcy with serine to form cystathionine.

3.Cleavage Phase‌: CBL then cleaves the cystathionine molecule to regenerate Hcy.

This cyclic amplification strategy enables highly sensitive quantitative Hcy determination. Consequently, CBL serves as the cornerstone enzyme in cystathionine-based Hcy detection kit development while also finding utility in other enzymatic applications requiring β-specific cleavage catalysis.

Properties

Isoelectric point: 6.0
Michaelis constant: 2.8×10^-4mol/L (L-cystathionine)
Optimum pH: 8.0                     Fig. 1
Optimum temperature: 40℃                     Fig. 2
pH Stability: 6.0~9.0 (25℃, 24hr)                     Fig. 3
Thermal stability: <50℃ (pH 7.4, 30min)                     Fig. 4
Inhibitors: Cu2+
Effect of various chemicals:                     Table 1
Reaction：

Specifications

Product Name

Cystathionine-β-Lyase (CBL), CAS No.9055-05-4

Synonyms

Cystathionine beta-lyase | Cystathionine betalyase | Cystathionine β-lyase | METC

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), ≥90 U/mg protein

Bioactivity

≥90 U/mg protein; Unit definition: One unit of activity is defined as the production of 1μmol of L-homocysteine per min at 37℃ at pH8.0.

Accession #

F5A553

SDS-PAGE

45 kDa (SDS-PAGE)

CAS

9055-05-4

Enzyme Commission Number

EC 4.4.1.8

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥90 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.Store in the dark. Desiccated.