The core of carbon-nitrogen metabolic crosstalk lies in the dynamic conversion between carbon skeletons and nitrogen flow. Glutamate and α-ketoglutarate form key hubs connecting the TCA cycle, transamination reactions, glutamine metabolism, and amino acid biosynthesis. Their changes reflect not ...
Topic
α-ketoglutarate
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...
Isocitrate dehydrogenase (IDH) is a key enzyme family involved in the tricarboxylic acid cycle, NADPH supply, α-ketoglutarate generation, and cellular redox homeostasis. In mammalian cells, the major isoenzymes include IDH1, IDH2, and IDH3. IDH1 and IDH2 are NADP⁺-dependent enzymes and are ...
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Techniques often explored alongside immunological experiments.