Folinic acid - Moligand™, 10mM in DMSO , CAS No.58-05-9

CAS: 58-05-9 Cat. No.: F424873 Molecular Weight: 473.44 EC Number: 216-082-8,200-361-6,846-741-0

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

folinic acid|leucovorin|5-Formyltetrahydrofolic acid|folinate|58-05-9|Welcovorin|Leucal|Acide folinique|Folinic acid-SF|Leucoverin|5-Formyl-5,6,7,8-tetrahydrofolic acid|N5-Formyltetrahydrofolic acid|UNII-Q573I9DVLP|Q573I9DVLP|5-formyltetrahydrofolate|CHEB

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Folinic acid Folinic acid (Leucovorin) is a biological folic acid that increases the level of reduced folates in tissues, which promotes the inhibition of thymidylate synthase (TS) .

Targets

Thymidylate Synthase

Specifications

Synonyms

folinic acid | leucovorin | 5-Formyltetrahydrofolic acid | folinate | 58-05-9 | Welcovorin | Leucal | Acide folinique | Folinic acid-SF | Leucoverin | 5-Formyl-5, 6, 7, 8-tetrahydrofolic acid | N5-Formyltetrahydrofolic acid | UNII-Q573I9DVLP | Q573I9DVLP | 5-formyltetrahydrofolate | CHEB

Specifications & Purity

Moligand™, 10mM in DMSO

Biochemical and Physiological Mechanisms

Folinic acid (Leucovorin) is a biological folic acid that increases the level of reduced folates in tissues, which promotes the inhibition of thymidylate synthase (TS).

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Product Properties

• pKa: pKₐ: 0.99 (Predicted), pKₐ: 3.1, pKₐ: 2.8, pKₐ: 10.4, pKₐ: 9.74 (Predicted)
• Ki Data: Dihydrofolate reductase: Ki= 2.37 μM (human); CEM: Ki= 0.74 μM (human)

Names and Identifiers

• Canonical Smiles: C1C(N(C2=C(N1)N=C(NC2=O)N)C=O)CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)O)C(=O)O
• IUPAC Name: (2S)-2-[[4-[(2-amino-5-formyl-4-oxo-3,6,7,8-tetrahydropteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
• InChIKey: VVIAGPKUTFNRDU-ABLWVSNPSA-N
• INCHI: 1S/C20H23N7O7/c21-20-25-16-15(18(32)26-20)27(9-28)12(8-23-16)7-22-11-3-1-10(2-4-11)17(31)24-13(19(33)34)5-6-14(29)30/h1-4,9,12-13,22H,5-8H2,(H,24,31)(H,29,30)(H,33,34)(H4,21,23,25,26,32)/t12?,13-/m0/s1
• Isomeric SMILES: C1C(N(C2=C(N1)N=C(NC2=O)N)C=O)CNC3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
• Alternate CAS: 58-05-9,1492-18-8,41927-89-3,6035-45-6 (calcium (1:1) salt,penta-hydrate)
• MeSH Entry Terms: 5 Formyltetrahydrofolate;5 Formyltetrahydropteroylglutamate;5-Formyltetrahydrofolate;5-Formyltetrahydropteroylglutamate;Acid, Folinic;Calcium Folinate;Calcium Leucovorin;Citrovorum Factor;Factor, Citrovorum;Folinate, Calcium;Folinic Acid;Folinic Acid SF;F
• Molecular Weight: 473.44
• Reaxy-Rn: 5324589
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5324589&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Glutamic acid and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Glutamic acid and derivatives
• Alternative Parents: N-acyl-alpha amino acids Hippuric acids Pterins and derivatives Aminobenzamides Phenylalkylamines Aniline and substituted anilines Benzoyl derivatives Secondary alkylarylamines Hydroxypyrimidines Dicarboxylic acids and derivatives Tertiary carboxylic acid amides Heteroaromatic compounds Secondary carboxylic acid amides Amino acids Carboxylic acids Azacyclic compounds Carbonyl compounds Hydrocarbon derivatives Organic oxides Organopnictogen compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Glutamic acid or derivatives - Hippuric acid or derivatives - Hippuric acid - N-acyl-alpha-amino acid - N-acyl-alpha amino acid or derivatives - Pterin - Aminobenzamide - Aminobenzoic acid or derivatives - Pteridine - Benzamide - Benzoic acid or derivatives - Benzoyl - Phenylalkylamine - Aniline or substituted anilines - Secondary aliphatic/aromatic amine - Hydroxypyrimidine - Benzenoid - Pyrimidine - Monocyclic benzene moiety - Dicarboxylic acid or derivatives - Tertiary carboxylic acid amide - Heteroaromatic compound - Secondary carboxylic acid amide - Carboxamide group - Amino acid - Organoheterocyclic compound - Secondary amine - Azacycle - Carboxylic acid - Organic oxygen compound - Carbonyl group - Amine - Organic oxide - Organic nitrogen compound - Organopnictogen compound - Organooxygen compound - Organonitrogen compound - Hydrocarbon derivative - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as glutamic acid and derivatives. These are compounds containing glutamic acid or a derivative thereof resulting from reaction of glutamic acid at the amino group or the carboxy group, or from the replacement of any hydrogen of glycine by a heteroatom.
• External Descriptors: formyltetrahydrofolic acid

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Refractive Index: n20D1.75 (Predicted)
• Specific Rotation[α]: α25/D +16.76°, c = 3.5 in 5% sodium carbonate; α21/D +14.9°, c = 1 in water; α20/D -15.1° , c = 1.82; α 20/D +14.26°
• Melt Point(°C): 240-250° C
• Molecular Weight: 473.400 g/mol
• XLogP3: -1.200
• Hydrogen Bond Donor Count: 7
• Hydrogen Bond Acceptor Count: 10
• Rotatable Bond Count: 9
• Exact Mass: 473.166 Da
• Monoisotopic Mass: 473.166 Da
• Topological Polar Surface Area: 216.000 Ų
• Heavy Atom Count: 34
• Formal Charge: 0
• Complexity: 911.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 1
• Undefined Atom Stereocenter Count: 1
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Nan Jiang, Xuanwei Ding, Yuan Lu.  (2020)  Development of a robust Escherichia coli-based cell-free protein synthesis application platform.  BIOCHEMICAL ENGINEERING JOURNAL,      [PMID:33100890] [10.1016/j.bej.2020.107830]

2. Lekang Liu, Mingbo Shao, Luoyuan Guo, Wenjun Wang, Xiuwen Zheng, Xiaolei Jiang.  (2025)  Cu-Doped MnO2 Nanoparticles Loaded with Docetaxel Synergistically Enhance Chemodynamic Therapy through Ferroptosis and Cuproptosis.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.4c06487]

3. Zhou Yang, Lingling Dai, Fangfei Liu, Kun Jia, Zhiyuan Ren, Yanmei Yang, Qingmeng Zhang, Weifeng Li, Ming Chen.  (2025)  Thermostable CNTs@Au NPs nanocomposites for high-temperature assisted SERS molecular fingerprinting of two natural folates.  ANALYTICA CHIMICA ACTA,      [PMID:41135988] [10.1016/j.aca.2025.344678]