Glycerol Dehydrogenase (GYD), CAS No.9028-14-2

CAS: 9028-14-2 Cat. No.: G774082 EC Number: 232-824-3

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥80%(SDS-PAGE), ≥150 U/mg protein

Accession #

P50173

Expression system

E. coli

Bioactivity

≥150 U/mg protein; Unit definition: One unit causes the formation of one micromole of NADH per min at pH 10.0 at 25℃.

Overview

Source: Microorganism 

Isoelectric point: 5.4 

Michaelis constant: 1.5×10^-3 M (Glycerol) ; 2.7×10^-3 M (NAD) 

Optimum pH: 10.0~12.0                     Fig. 1

Optimum temperature: 50℃                     Fig. 3

pH Stability: 8.5~11.0 (25℃, 20hr)                     Fig. 2

Thermal stability: 35℃~60℃ (pH 7.5, 15min)                     Fig. 4

Inhibitors: Cu2+, Fe3+, Mg2+, Ni2+, NEM, EDTA, Proclin, SDS 

Effect of various chemicals:                     Table 1

Reaction：

Specifications

Product Name

Glycerol Dehydrogenase (GYD), CAS No.9028-14-2

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥80%(SDS-PAGE), ≥150 U/mg protein

Bioactivity

≥150 U/mg protein; Unit definition: One unit causes the formation of one micromole of NADH per min at pH 10.0 at 25℃.

Accession #

P50173

SDS-PAGE

41 kDa (SDS-PAGE)

CAS

9028-14-2

Enzyme Commission Number

EC 1.1.1.6

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥150 U/mg protein

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ (1 year). Upon delivery aliquot. Store in the dark. Desiccated.