I-A/I-E Rat mAb (PE-Cy7) - Primary antibody, specific to I-A/I-E

Cat. No.: Ab190260

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

M5/114

Conjugation

PE-Cy7

Overview

Rat anti Mouse I-A/I-E Antibody, Monoclonal (M5/114), could be used for Flow Cytometry.

Specifications

Product Name

I-A/I-E Rat mAb (PE-Cy7) - Primary antibody, specific to I-A/I-E

Synonyms

IAb | Ia2 | Ia-2 | Abeta | H-2Ab | H2-Ab | Rmcs1 | I-Abeta | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides

Host species

Rat

Specificity

I-A/I-E

Immunogen

Activated C57BL/6 mouse spleen cells

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

PE-Cy7

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

774nm

Ex/Em(nm)

Ex:565nm;Em:774nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: I-A/I-E Rat mAb (PE-Cy7) (Ab190260)

-A/I-E Rat mAb (PE-Cy7) (Ab190260) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with I-A/I-E Rat mAb (PE-Cy7) (Ab190260) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Flow Cytometry: I-A/I-E Rat mAb (PE-Cy7) (Ab190260)

I-A/I-E Rat mAb (PE-Cy7) (Ab190260) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with I-A/I-E Rat mAb (PE-Cy7) (Ab190260) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

HLA-DQB1 Tbio HLA class II histocompatibility antigen, DQ beta 1 chain (0 Activities)

Discover Target: HLA-DQB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)