GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)Protein concentration: See COA; ≥5 U/mg protein
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),Protein concentration: See COA; ≥5 U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
L-fucokinase/GDP-fucose pyrophos-phorylase (FKP, E.C. 2.7.1.52/2.7.7.30), derived from Bacteroides fragilis.This bifunctional enzyme catalyzes the phosphorylation of L-fucose to form Fuc-1-P, and further converts Fuc-1-P to GDP-Fuc, which serves as a fucosyl donor for glycosyltransferases. It acts as a tool enzyme for the synthesis of GDP-Fuc and its derivatives. This product is recombinantly expressed in Escherichia coli, featuring high purity and good compatibility, suitable for one-pot multi-enzyme systems.
Reaction:
Protocol for Use
Add substrates to the reaction mixture, supplemented with 5 mM MgCl₂. An appropriate buffer (e.g., Tris-HCl) may be added to adjust the pH to 7.5–8.5, followed by the addition of an appropriate amount of FKP (adjust according to reaction requirements).
Mix thoroughly and incubate the sample at 37°C for 30–60 minutes. The enzyme exhibits maximum activity in near-neutral pH buffers. The recommended reaction buffer is Tris-HCl (pH 8.0); other common biological buffers such as Tris or PBS are also applicable.
Note: For scaled-up reactions, adjust the incubation time or enzyme amount based on actual conditions. Gentle shaking may be used for mixing. Addition of inorganic pyrophosphatase can improve the conversion rate.
Precautions
For your safety and health, please wear lab coats and disposable gloves when handling. This product is for research use only!
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), Protein concentration: See COA; ≥5 U/mg protein
Biochemical and Physiological Mechanisms
Bifunctional enzyme involved in the salvage pathway of GDP-fucose synthesis. Catalyzes two successive reactions, the ATP-dependent phosphorylation of L-fucose to L-fucose 1-phosphate, and its guanylylation to GDP-L-fucose. GDP-fucose is an important fucos
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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