Mutant EMSA Probe HIF-1α - 10μM

Cat. No.: M745636

GRADE & PURITY 10μM

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Aladdin's EMSA Mutant Probe - HIF-1α is a mutant of the HIF-1α consensus oligonucleotide designed for EMSA . It can be used as a negative control of EMSA Probe - HIF-1α in cold mutant probe competition reactions.The nucleotide sequence of EMSA Mutant Probe - HIF-1α is as follows

Precautions：

Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Set up the cold mutant probe competition reaction by adding the reagents in sequence as follows:Nuclease-free Water 4µlEMSA/Gel-Shift Binding Buffer (5X) 2µlNuclear proteins or purified transcription factors 2µlUnlabeled mutant probe 1µlLabeled probe 1µlTotal volume10µl Note: If a larger volume of unlabeled mutant probe is used, the volume of Nuclease-free Water should be reduced accordingly to keep the total reaction volume at 10µl.

Specifications

Specifications & Purity

10μM

Stability And Storage

Store at -20℃ for up to 1 year.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.