NGS Barcode Adapters 1-16 Kit for Ion Torrent - 16×10μl, high purity

Cat. No.: N670210

Overview

Product content:

N670210	Component	16×10 μL	Storage
N670210A	Ion P1 Adapter (10 μM)	160 μL	-20℃. Avoid freeze/thaw cycle.
N670210B	Ion Barcode Ax (10 μM)	16×10 μL	-20℃. Avoid freeze/thaw cycle.

Product Introduction:

NGS Barcode Adapter for Ion Torrent (1-16) is a specialized kit designed for library construction on Ion Torrent high-throughput sequencing platforms. It can be used to construct libraries suitable for Ion PGM ™ System, Ion Proton System, Ion S5 System, and Ion GeneStudio ™ Multi sample targeted sequencing DNA library using Ion Torrent high-throughput sequencing platforms such as S5 Series System. This kit contains 16 different barcode adapters, including P1 Adaptor and Barcode A1 to Barcode A16, used in the library construction of the Ion Torrent sequencing platform. It is compatible with the Kangwei Century second-generation sequencing rapid DNA library construction kit and the AmpliSeq second-generation sequencing DNA library construction kit and can be used to construct 16 index labeled DNA libraries for sequencing analysis. All reagents provided in the kit have undergone strict quality control and functional validation, ensuring the reliability of sample identification, stability and repeatability of library construction to the greatest extent possible.

Notice:

1. The adapter is a double chain structure, please do not place it above room temperature to avoid chain breakage and affect use.

2. Before opening the barcode adapter, please centrifuge briefly to collect the liquid at the bottom of the tube to avoid cross contamination between different barcode adapters.

Operation steps
The packaging quantity of each Barcode Adapter in this kit is sufficient for library construction of ≤ 14 pieces of 100 ng Input DNA or 1 piece of 1 µ g Input DNA. The amount of adapter used can be adjusted according to the initial amount of DNA in the sample to achieve optimal usage effect. The following operation example is performed using the Kangwei Century second-generation sequencing rapid DNA library kit:

 1. Add the following reagents sequentially into a new 0.2 ml PCR tube and mix thoroughly.

Component	Volume	Volume
	50-100-ng DNA Input	1-µg DNA Input
End Repaired DNA Fragment	60 μl	60 μl
Ligation and Nick Repair Buffer	10 μl	10 μl
Ion P1 Adapter	7 μl(1 μM )	7 μl(10 μM )
Ion Barcode Ax	7 μl(1 μM )	7 μl(10 μM )
Nuclease-free Water	12 μl	10 μl
DNA Ligase	2 μl	4 μl
Bst DNA Polymerase	2 μl	2 μl
Total volume	100 μl	100 μl

Attention: It is recommended that the molar ratio of the amount of Adaptor added to the DNA fragment be 10:1-20:1. Please refer to the instructions for using the Kangwei Century second-generation sequencing rapid DNA library kit for specific concentrations and amounts of Adaptor. When the DNA amount is 10-100 ng, the recommended concentration of Adaptor is 1 μ M (less than 260 bp) or 0.5 μ M (300-400 bp); When the DNA content is 1 µ g, Adaptor recommends using a concentration of 10 μ M (less than 260 bp) or 5 μ M (300-400 bp).
2. Place the PCR tube in the PCR instrument, set the hot cap to 80 ℃, and run the following program:
 

Stage	Temperature	Time
Hold	25℃	15 minutes
Hold	65℃	5 minutes
Hold	4℃	Hold

Attention: Please proceed to the next step as soon as possible after connecting the connector to purify the product of the connector connection.

Sequence information
  Ion P1 Adapter：5’-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3’ Ion Barcode Ax：5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXXGAT-3’ *The underlined sequence X is the barcode sequence used during sequencing.
 The sequence of barcodes corresponding to each Barcode Adapter is as follows:
 

Component	Barcode
Ion Barcode A1	CTAAGGTAAC
Ion Barcode A2	TAAGGAGAAC
Ion Barcode A3	AAGAGGATTC
Ion Barcode A4	TACCAAGATC
Ion Barcode A5	CAGAAGGAAC
Ion Barcode A6	CTGCAAGTTC
Ion Barcode A7	TTCGTGATTC
Ion Barcode A8	TTCCGATAAC
Ion Barcode A9	TGAGCGGAAC
Ion Barcode A10	CTGACCGAAC
Ion Barcode A11	TCCTCGAATC
Ion Barcode A12	TAGGTGGTTC
Ion Barcode A13	TCTAACGGAC
Ion Barcode A14	TTGGAGTGTC
Ion Barcode A15	TCTAGAGGTC
Ion Barcode A16	TCTGGATGAC

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads