Pectolyase from Aspergillus japonicus

Cat. No.: P755148

GRADE & PURITY lyophilized powder,≥0.3 units/mg solid

Overview

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.

Application:

Pectolyase from Aspergillus japonicus has been used:

• in the lysis of chlorella cells for protoplast generation
• as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
• to induce reorganization of Arabidopsis root cap cell architecture

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Specifications

Product Name

Pectolyase from Aspergillus japonicus

Specifications & Purity

lyophilized powder, ≥0.3 units/mg solid

Biochemical and Physiological Mechanisms

Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, end

Molecule Type

Enzyme

Storage and Shipping

Concentration

lyophilized powder,≥0.3 units/mg solid

Storage

Store at 2-8°C

Shipped In

Wet ice