Piperine - Moligand™, 10mM in DMSO , CAS No.94-62-2

CAS: 94-62-2 Cat. No.: P407794 Molecular Weight: 285.34 Beilstein Registry Number: 90741 EC Number: 202-348-0

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

1-Piperoylpiperidine | 2,4-Pentadien-1-one, 5-(1,3-benzodioxol-5-yl)-1-(1-piperidinyl)-, (2E,4E)-

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Piperine (1-Piperoylpiperidine) is the alkaloid responsible for the pungency of black pepper and long pepper, which has also been used in some forms of traditional medicine and as an insecticide.
In vitro

Piperine (1-Piperoylpiperidine) is the alkaloid responsible for the pungency of black pepper and long pepper, along with chavicine (an isomer of piperine). It has also been used in some forms of traditional medicine and as an insecticide. Piperine has been found to inhibit human CYP3A4 and P-glycoprotein, enzymes important for the metabolism and transport of xenobiotics and metabolites. Piperine is found to be cytotoxic towards DLA and EAC cells at a concentration of 250 μ g/ml. Piperine (1.14 mg/dose/animal) could inhibit the solid tumor development in mice induced with DLA cells and increase the life span of mice bearing Ehrlich ascites carcinoma tumor.

In vivo

LD50: Mice 15.1mg/kg (i.v.), 43mg/kg (i.p.), 200mg/kg (s.c.), 330mg/kg (i.g.); Rats 33.5mg/kg (i.p.), 514mg/kg (i.g.)
Cell Data

cell lines：

Concentrations：

Incubation Time：

Powder Purity:≥98%

Specifications

Synonyms

1-Piperoylpiperidine | 2, 4-Pentadien-1-one, 5-(1, 3-benzodioxol-5-yl)-1-(1-piperidinyl)-, (2E, 4E)-

Specifications & Purity

Moligand™, 10mM in DMSO

Biochemical and Physiological Mechanisms

Piperine (1-Piperoylpiperidine) is the alkaloid responsible for the pungency of black pepper and long pepper, which has also been used in some forms of traditional medicine and as an insecticide.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Names and Identifiers

• Isomeric SMILES: C1CCN(CC1)C(=O)/C=C/C=C/C2=CC3=C(C=C2)OCO3
• WGK Germany: 3
• RTECS: TN2321500
• Molecular Weight: 285.34
• Beilstein: 90741
• Reaxy-Rn: 90739
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=90739&ln=

Associated Targets(Human)

TRPV1 Tclin Transient receptor potential cation channel subfamily V member 1 (1 Activities)

Discover Target: TRPV1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAOB Tclin Amine oxidase [flavin-containing] B (1 Activities)

Discover Target: MAOB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro DMSO: 56 mg/mL warmed with 50ºC Water: bath (196.94 mM); Ethanol: 11 mg/mL warmed with 50ºC Water: bath (38.68 mM); Water: Insoluble
• Melt Point(°C): 128-132°C

References

1. Shuai Chen, Yanhui Zhang, Jing Qing, Yahong Han, David Julian McClements, Yanxiang Gao.  (2020)  Core-shell nanoparticles for co-encapsulation of coenzyme Q10 and piperine: Surface engineering of hydrogel shell around protein core.  FOOD HYDROCOLLOIDS,      [PMID:] [10.1016/j.foodhyd.2020.105651]

2. Miao Zhong, Lingzhen Chen, Yue Tao, Jintao Zhao, Bingbing Chang, Fang Zhang, Jingwen Tu, Wenqing Cai, Baoxin Zhang.  (2023)  Synthesis and evaluation of Piperine analogs as thioredoxin reductase inhibitors to cause oxidative stress-induced cancer cell apoptosis.  BIOORGANIC CHEMISTRY,      [PMID:37320912] [10.1016/j.bioorg.2023.106589]

3. Ligang Yu, Xiaoyue Zhang, Wenyan Sun, Guang Shen, Yukun Yang, Maomao Zeng.  (2023)  The influence of piperine on oxidation-induced porcine myofibrillar protein gelation behavior and fluorescent advanced glycation end products formation in model systems.  FOOD CHEMISTRY,      [PMID:37060667] [10.1016/j.foodchem.2023.136119]

4. Ning Jia, Pengfei Guo, Kaiyuan Zhang, Chen Liu, Ruihao Chen, Zhe Liu, Qian Ye, Hongqiang Wang.  (2022)  Defect Passivation by Natural Piperine Molecule Enabling for Stable Perovskite Solar Cells with Efficiencies over 23%.  ACS Sustainable Chemistry & Engineering,      [PMID:] [10.1021/acssuschemeng.2c05527]

5. Ligang Yu, Yong Li, Yukun Yang, Caixia Guo, Meiping Li.  (2022)  Inhibitory effects of curcumin and piperine on fluorescent advanced glycation end products formation in a bovine serum albumin–fructose model.  INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY,  57  (7): (4646-4655).  [PMID:] [10.1111/ijfs.15804]

6. Song Lingyu, Wang Yan, Zhen Yunhuan, Li Dengke, He Xidong, Yang Hong, Zhang Huayang, Liu Qi.  (2020)  Piperine inhibits colorectal cancer migration and invasion by regulating STAT3/Snail-mediated epithelial–mesenchymal transition.  BIOTECHNOLOGY LETTERS,  42  (10): (2049-2058).  [PMID:32500474] [10.1007/s10529-020-02923-z]

7. Liu Kai, Liu Huijun, Li Zhendong, Li Wei, Li Liuxing.  (2020)  In vitro dissolution study on inclusion complex of piperine with ethylenediamine-β-cyclodextrin.  JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY,  96  (3): (233-243).  [PMID:] [10.1007/s10847-020-00980-5]

8. Hongyan He, Qi Zhang, Jian-Rong Wang, Xuefeng Mei.  (2017)  Structure, physicochemical properties and pharmacokinetics of resveratrol and piperine cocrystals.  CRYSTENGCOMM,  19  (41): (6154-6163).  [PMID:] [10.1039/C7CE01468F]

9. Li Wanqian, Whiteway Malcolm, Hang Sijin, Yu Jinhua, Lu Hui, Jiang Yuanying.  (2025)  Impairing the interaction between Erg11 and cytochrome P450 reductase Ncp1 enhances azoles’ antifungal activities.  Nature Communications,  16  (1): (1-18).  [PMID:40707518] [10.1038/s41467-025-62131-z]

10. Jian-Guo Zhang, Hao-Xiang Wu, Ai Zhang, Kiran Thakur, Fei Hu, Zhao-Jun Wei.  (2025)  Comprehensive analysis of the impact of extraction methods on the volatile organic compounds and flavor profiles of Piper nigrum L. varieties.  FOOD CHEMISTRY,      [PMID:40645050] [10.1016/j.foodchem.2025.145425]

11. Di Wu, Xia Hu, Zhangyu Cai, Jing Zhang, Fang Geng, Hui Li.  (2023)  Binding behavior and antioxidant study of spice extract piperine with respect to meat myoglobin.  Food & Function,  14  (14): (6422-6431).  [PMID:37395089] [10.1039/D3FO00617D]

12. Mengmeng Wang, Bin Yang, Zongyi Ren, Jietao Liu, Chenwang Lu, Haifeng Jiang, Fei Ling, Gaoxue Wang, Tianqiang Liu.  (2022)  Inhibition of the largemouth bass virus replication by piperine demonstrates potential application in aquaculture.  JOURNAL OF FISH DISEASES,  46  (3): (261-271).  [PMID:36504104] [10.1111/jfd.13740]

13. Zhang Chaohua, Gu Fenglin, Hu Weicheng, Wu Guiping, Chen Weijun, Dong Conghui, Niu Zhiqiang.  (2022)  Effect of extraction technique on chemical compositions and antioxidant activities of freeze-dried green pepper.  Frontiers in Nutrition,      [PMID:36118756] [10.3389/fnut.2022.998840]

14. Rui Li, Boyu Pan, Zeyang Liu, Runfang Wang, Xiaofeng Wang, Shihui Cao, Jie Zhang, Bao Jin, Liren Liu, Chunnuan Wu.  (2025)  Elucidating the molecular mechanisms of the mongolian medicine Ana Gaqi Nari in gastric cancer treatment through a multi-omics-guided experimental validation.  PHYTOMEDICINE,      [PMID:41330183] [10.1016/j.phymed.2025.157623]

15. Xue He, Yinting Lu, Hao Zhang, Bing Lin, Huijiao Lu, Zhaoan Yu, Yan Li, Qing Xi, Rongxin Zhang.  (2025)  Piperine Inhibits the Immune Checkpoint CD47 to Enhance Immunity Against Pancreatic Cancer.  PHYTOTHERAPY RESEARCH,      [PMID:41253721] [10.1002/ptr.70110]

16. Chang Liu, Zhilin Hao, Ziming Wang, Kun Cui, Chen Zhang, Jingrong Wang, Lulu Guo, Fuqiang Liu, Qixin Deng, Zi Yang, Xudong Chen, Maojun Yang, Jianping Xie, Sensen Zhang, Jian Mao.  (2026)  Structure of the Insect Alkaline Taste Receptor Alka and Insights into Its Alkaline Ligand Recognition Mechanism.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:41601143] [10.1021/acs.jafc.5c13948]

17. Yali Yang, Hui Ding, Kaijun Quan, Hongwei Zhao, Mingxia Sun, Zhimin Yang, Xiuhui Liu, Hongdeng Qiu, Jia Chen.  (2026)  Pyrene trisulfonic acid-functionalized silica-based multi-mode stationary phase for separation of hydrophilic, hydrophobic compounds and rare earth ions.  ANALYTICA CHIMICA ACTA,      [PMID:41813355] [10.1016/j.aca.2026.345267]