Polymyxin B (PMB) Enzyme

Cat. No.: rp216623

GRADE & PURITY BioReagent, EnzymoPure™, Sterile

Overview

Polymyxin B enzyme is a water-soluble protein using recombinant genes and is a dark brown liquid. Recombinant gene protein based on the chemical structure of polymyxin B. The active center of its gene fragment can destroy the chemical structural formula of polymyxin B, so that the antimicrobial properties of the drug are lost after ring-opening/chain breaking.
When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly.
Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane.
During sterility test, take 3ml of enzyme and add 2ml of sterile water and shake well to make diluent of polymyxin B enzyme. Take 2ml of enzyme diluent and add 1500ml of rinsing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. Use a manual syringe to Pierce the respiratory mouth of the three incubators, add 1ml of diluent enzyme to each of them, spread the enzyme on the entire surface of the filter membrane as much as possible, rest for 10 minutes, and make the high concentration of enzyme fully contact with the filter membrane of the incubator, so as to destroy (neutralize and inactivate) the residual polymyxin B drug on the filter membrane, and then pump into the corresponding medium and shake well. Positive pairs were treated with 1ml of E. coli (100CFU/ml).
Adding 2ml dilution of polymyxin B enzyme to the rinse solution can remove a small amount of antimicrobial activity of polymyxin B remaining in the filter membrane.
Adding 1ml dilution of polymyxin B enzyme to three incubators can remove a small amount of antimicrobial activity of polymyxin B remaining on the inner wall of the incubators and on the surface of the filter membrane.
Shake the positive pair gently once a day in the morning and afternoon.
Customers can do methodological verification according to the above, but also according to the actual operation of the verification.

Specifications

Product Name

Polymyxin B (PMB) Enzyme

Synonyms

Polymyxin B (PMB) Enzyme

Grade

BioReagent, Sterile, EnzymoPure™

Specifications & Purity

EnzymoPure™, BioReagent, sterile

Molecule Type

Enzyme

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (12 months). Upon receipt, it is recommended to aliquot.