Pyridoxal 5′-phosphate hydrate - ≥98% , CAS No.853645-22-4

CAS: 853645-22-4 Cat. No.: P136795 Molecular Weight: 247.14 (anhydrous basis) Beilstein Registry Number: 234749 EC Number: 200-208-3
AVAILABLE TO ORDER
GRADE & PURITY ≥98%
Synonyms
Vitazechs | FT-0631236 | P5P | P-5'-P | Pyromijin | 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate | CCG-266929 | EX-A980 | MC-1 | Pyridoxal 5/'-phosphate (hydrate) | SGCUT00188 | VITAMIN B6 (PYRIDOXAL 5-PHOSPHATE) | 3-hydroxy-2-methyl
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
1g
P136795-1g
1
$9.90
5g
P136795-5g
2
$20.90
25g
P136795-25g
3
$91.90
100g
P136795-100g
1
$339.90
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Why this grade

≥98% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 18 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

 Product Description:

Pyridoxal 5′-phosphate (PLP) is synthesized in a multiple-step process. The two pathways inlcude pyridoxal phosphate biosynthetic protein (PdxA)- pyridoxine-5′-phosphate synthase (PdxJ) pathway and the pyridoxal 5′-phosphate synthase subunit PDX1/PDX2 pathway. It is the active form of pyridoxine.


 Product Application:

Pyridoxal 5′-phosphate hydrate has also been used: as a reference standard to quantify vitamin B6 in feed and digesta samples using high performance liquid chromatography (HPLC); in D-amino acid transaminase reaction(10);as a cofactor for L-glutamic acid decarboxylase

Specifications

Synonyms
Vitazechs | FT-0631236 | P5P | P-5'-P | Pyromijin | 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate | CCG-266929 | EX-A980 | MC-1 | Pyridoxal 5/'-phosphate (hydrate) | SGCUT00188 | VITAMIN B6 (PYRIDOXAL 5-PHOSPHATE) | 3-hydroxy-2-methyl
Specifications & Purity
≥98%
Biochemical and Physiological Mechanisms
Pyridoxal 5′-phosphate is used as a cofactor for a wide range of enzymes including mitochondrial cysteine desulfurase, cystathionine γ-synthase (CGS), ornithine 4, 5-aminomutase (OAM), and d-serine dehydratase. PLP is used in the studies of PLP-dependent e
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Purity
≥98%
Names and Identifiers
Canonical SmilesCC1=NC=C(C(=C1O)C=O)COP(=O)(O)O
IUPAC Name(4-formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate
InChIKeyNGVDGCNFYWLIFO-UHFFFAOYSA-N
INCHI1S/C8H10NO6P/c1-5-8(11)7(3-10)6(2-9-5)4-15-16(12,13)14/h2-3,11H,4H2,1H3,(H2,12,13,14)
Isomeric SMILES CC1=NC=C(C(=C1O)C=O)COP(=O)(O)O
WGK Germany 3
Molecular Weight 247.14 (anhydrous basis)
Beilstein 234749
Reaxy-Rn 234749
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=234749&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassOrganoheterocyclic compounds
ClassPyridines and derivatives
SubclassPyridine carboxaldehydes
Intermediate Tree Nodes Not available
Direct ParentPyridoxals and derivatives
Alternative Parents Monoalkyl phosphates  Methylpyridines  Hydroxypyridines  Aryl-aldehydes  Vinylogous acids  Heteroaromatic compounds  Azacyclic compounds  Organopnictogen compounds  Organonitrogen compounds  Organic oxides  Hydrocarbon derivatives  
Molecular FrameworkAromatic heteromonocyclic compounds
Substituents Pyridoxal - Aryl-aldehyde - Monoalkyl phosphate - Hydroxypyridine - Methylpyridine - Organic phosphoric acid derivative - Phosphoric acid ester - Alkyl phosphate - Vinylogous acid - Heteroaromatic compound - Azacycle - Organopnictogen compound - Aldehyde - Organic oxygen compound - Organic nitrogen compound - Organooxygen compound - Organonitrogen compound - Hydrocarbon derivative - Organic oxide - Aromatic heteromonocyclic compound
DescriptionThis compound belongs to the class of organic compounds known as pyridoxals and derivatives. These are compounds containing a pyridoxal moiety, which consists of a pyridine ring substituted at positions 2,3,4, and 5 by a methyl group, a hydroxyl group, a carbaldehyde group, and a hydroxymethyl group, respectively.
External Descriptors monohydroxypyridine - pyridinecarbaldehyde - methylpyridines - vitamin B6 phosphate
3D Structure
Interactive Chemical Structure Model





Associated Targets(Human)
P2RX1 Tchem P2X purinoceptor 1 (1 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
F2 Tclin Thrombin (11687 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ESR1 Tclin Estrogen receptor alpha (17718 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PGR Tclin Progesterone receptor (8562 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CHRM2 Tclin Muscarinic acetylcholine receptor M2 (10671 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HTR1A Tclin Serotonin 1a (5-HT1a) receptor (14969 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ADRA2A Tclin Alpha-2a adrenergic receptor (9450 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AR Tclin Androgen Receptor (11781 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CHRM1 Tclin Muscarinic acetylcholine receptor M1 (12690 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
MAOA Tclin Monoamine oxidase A (11911 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
DRD1 Tclin Dopamine D1 receptor (9720 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ACHE Tclin Acetylcholinesterase (18204 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PTGS1 Tclin Cyclooxygenase-1 (9233 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SLC6A2 Tclin Norepinephrine transporter (10102 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SLC6A4 Tclin Serotonin transporter (12625 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ADRA1A Tclin Alpha-1a adrenergic receptor (8359 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
OPRM1 Tclin Mu opioid receptor (19785 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
DRD3 Tclin Dopamine D3 receptor (14368 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SLC6A3 Tclin Dopamine transporter (10535 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
KCNH2 Tclin HERG (29587 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PDE3A Tclin Phosphodiesterase 3A (3309 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HTR2B Tclin Serotonin 2b (5-HT2b) receptor (10323 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TBXA2R Tclin Thromboxane A2 receptor (5717 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PDE4A Tclin Phosphodiesterase 4A (1943 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ADORA3 Tchem Adenosine A3 receptor (15931 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HRH3 Tclin Histamine H3 receptor (10389 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
P2RX4 Tchem P2X purinoceptor 4 (516 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
P2RX1 Tchem P2X purinoceptor 1 (328 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
KDR Tclin Vascular endothelial growth factor receptor 2 (20924 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CAD Tchem Dihydroorotase (27 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PTPRB Tchem Receptor-type tyrosine-protein phosphatase beta (330 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ACP1 Tchem Low molecular weight phosphotyrosine protein phosphatase (1161 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
EHMT2 Tchem Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 (93046 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PMP22 Tbio Peripheral myelin protein 22 (699 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Associated Targets(non-human)
P2rx2 P2X purinoceptor 2 (106 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
P2rx3 P2X purinoceptor 3 (632 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Hdac6 Histone deacetylase 6 (222 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Rattus norvegicus (775804 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
rep Replicase polyprotein 1ab (378 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Trans-sialidase (51 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SARS-CoV-2 (38078 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

15 results found

Lot NumberCertificate TypeDateItem
E2413020Certificate of AnalysisFeb 05, 2026 P136795
F2520614Certificate of AnalysisJun 12, 2025 P136795
F2520629Certificate of AnalysisJun 12, 2025 P136795
F2520631Certificate of AnalysisJun 12, 2025 P136795
F2524735Certificate of AnalysisJun 12, 2025 P136795
D2321357Certificate of AnalysisFeb 11, 2025 P136795
J2424255Certificate of AnalysisOct 31, 2024 P136795
F2510672Certificate of AnalysisJul 10, 2024 P136795
F2510674Certificate of AnalysisJul 10, 2024 P136795
F2510673Certificate of AnalysisJul 10, 2024 P136795
F2510675Certificate of AnalysisJul 10, 2024 P136795
K2125309Certificate of AnalysisSep 11, 2023 P136795
K2125185Certificate of AnalysisSep 11, 2023 P136795
F1509085Certificate of AnalysisJun 09, 2023 P136795
B2208069Certificate of AnalysisAug 27, 2021 P136795

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Chemical and Physical Properties
Melt Point(°C)140-143°C
Molecular Weight247.140 g/mol
XLogP3-1.100
Hydrogen Bond Donor Count3
Hydrogen Bond Acceptor Count7
Rotatable Bond Count4
Exact Mass247.025 Da
Monoisotopic Mass247.025 Da
Topological Polar Surface Area117.000 Ų
Heavy Atom Count16
Formal Charge0
Complexity292.000
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count1
Documents & Articles
Citations of This Product
References
1. Hongpeng Wang, Mercy Vimbai Masuku, Yachen Tao, Jiayao Yang, Yi Kuang, Changjiang Lyu, Jun Huang, Shengxiang Yang.  (2023)  Improved Stability and Catalytic Efficiency of ω-Transaminase in Aqueous Mixture of Deep Eutectic Solvents.  MOLECULES,  28  (9): (3895).  [PMID:37175305] [10.3390/molecules28093895]
2. Xingchang Cha, Juanjuan Ding, Wenyan Ba, Shengping You, Wei Qi, Rongxin Su.  (2023)  High Production of γ-Aminobutyric Acid by Activating the xyl Operon of Lactobacillus brevis.  ACS Omega,      [PMID:36873027] [10.1021/acsomega.2c08272]
3. Lian Xu, Dan Nie, Bing-Mei Su, Xin-Qi Xu, Juan Lin.  (2022)  A chemoenzymatic strategy for the efficient synthesis of amphenicol antibiotic chloramphenicol mediated by an engineered L-threonine transaldolase with high activity and stereoselectivity.  Catalysis Science & Technology,  13  (3): (684-693).  [PMID:] [10.1039/D2CY01670B]
4. Sai Fang, Haoran Yu, Lanxin Xiao, Zhe Wang, Yixuan Lei, Gang Xu, Lirong Yang, Wenlong Zheng, Jianping Wu.  (2022)  Counteracting the Activity-Diastereoselectivity Trade-Off of l-Threonine Aldolase by Regulating the Proton Transfer Microenvironment.  ADVANCED SYNTHESIS & CATALYSIS,  364  (24): (4363-4370).  [PMID:] [10.1002/adsc.202201006]
5. Guozeng Wang, Zhihao Jiang, Qing Xiao, Chang Jiang, Xian'ai Shi.  (2022)  Visible spectrophotometric assay for characterization of ω-transaminases.  ANALYTICAL BIOCHEMISTRY,      [PMID:36208685] [10.1016/j.ab.2022.114933]
6. Li-Chao Wang, Lian Xu, Bing-Mei Su, Xin-Qi Xu, Juan Lin.  (2022)  An Effective Chemo-Enzymatic method with An Evolved L-Threonine Aldolase for Preparing L-threo-4-Methylsulfonylphenylserine Ethyl Ester of High Optical Purity.  Molecular Catalysis,      [PMID:] [10.1016/j.mcat.2022.112355]
7. Lichao Wang, Lian Xu, Bingmei Su, Wei Lin, Xinqi Xu, Juan Lin.  (2021)  Improving the Cβ Stereoselectivity of l-Threonine Aldolase for the Synthesis of l-threo-4-Methylsulfonylphenylserine by Modulating the Substrate-Binding Pocket To Control the Orientation of the Substrate Entrance.  CHEMISTRY-A EUROPEAN JOURNAL,  27  (37): (9654-9660).  [PMID:33843095] [10.1002/chem.202100752]
8. Hongjie Hui, Yajun Bai, Tai-Ping Fan, Xiaohui Zheng, Yujie Cai.  (2020)  Biosynthesis of Putrescine from L-arginine Using Engineered Escherichia coli Whole Cells.  Catalysts,  10  (9): (947).  [PMID:] [10.3390/catal10090947]
9. Li-Chao Wang, Lian Xu, Xin-Qi Xu, Bing-Mei Su, Juan Lin.  (2020)  An L-threonine aldolase for asymmetric synthesis of β-hydroxy-α-amino acids.  CHEMICAL ENGINEERING SCIENCE,      [PMID:] [10.1016/j.ces.2020.115812]
10. Lian Xu, Li-Chao Wang, Xin-Qi Xu, Juan Lin.  (2019)  Characteristics of L-threonine transaldolase for asymmetric synthesis of β-hydroxy-α-amino acids.  Catalysis Science & Technology,  (21): (5943-5952).  [PMID:] [10.1039/C9CY01608B]
11. Xiao-Ling Tang, Nan-Nan Zhang, Guo-Yan Ye, Yu-Guo Zheng.  (2019)  Efficient biosynthesis of (R)-3-amino-1-butanol by a novel (R)-selective transaminase from Actinobacteria sp..  JOURNAL OF BIOTECHNOLOGY,      [PMID:30853639] [10.1016/j.jbiotec.2019.02.008]
12. Zhang Zhi-Jun, Cai Rui-Feng, Xu Jian-He.  (2018)  Characterization of a new nitrilase from Hoeflea phototrophica DFL-43 for a two-step one-pot synthesis of (S)-β-amino acids.  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,  102  (14): (6047-6056).  [PMID:29744634] [10.1007/s00253-018-9057-7]
13. Qian Wang, Xiao-Mei He, Xi Chen, Gang-Tian Zhu, Ren-Qi Wang, Yu-Qi Feng.  (2017)  Pyridoxal 5′-phosphate mediated preparation of immobilized metal affinity material for highly selective and sensitive enrichment of phosphopeptides.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:28390667] [10.1016/j.chroma.2017.03.085]
14. Ailin Xiao, Jing Li, Tianjian Liu, Zhuxi Liu, Chuanfei Wei, Xiaomeng Xu, Qin Li, Jingxin Li.  (2016)  l-Cysteine enhances nutrient absorption via a cystathionine-β-synthase-derived H2S pathway in rodent jejunum.  CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,  43  (5): (562-568).  [PMID:26901099] [10.1111/1440-1681.12562]
15. S. Tang, D. Huang, N. An, D. Chen, D. Zhao.  (2016)  A novel pathway for the production of H2S by DAO in rat jejunum.  NEUROGASTROENTEROLOGY AND MOTILITY,  28  (5): (687-692).  [PMID:26813142] [10.1111/nmo.12765]
16. Binbin Chen, Jiahui Huang, Yingchun Liu, Lirong Yang, Qi Wang, Haoran Yu.  (2025)  Computational Study on the Catalytic Mechanism of UstD Catalyzing the Synthesis of γ-Hydroxy-α-Amino Acids.  Journal of Chemical Information and Modeling,      [PMID:39913657] [10.1021/acs.jcim.4c01998]
17. Ying-Ying Xu, Bin Zhang, Jie Bao.  (2025)  Production of γ-aminobutyric acid using corncob residue as carbohydrate feedstock by engineered Corynebacterium glutamicum.  BIOCHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.bej.2025.109629]
18. Tang Xiao-Ling, Liu Xiao, Suo Hui, Wang Zhi-Chao, Zheng Ren-Chao, Zheng Yu-Guo.  (2018)  Process development for efficient biosynthesis of l-DOPA with recombinant Escherichia coli harboring tyrosine phenol lyase from Fusobacterium nucleatum.  BIOPROCESS AND BIOSYSTEMS ENGINEERING,  41  (9): (1347-1354).  [PMID:29869726] [10.1007/s00449-018-1962-8]
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