GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5mg/mL
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide co
Host species
Rat
Specificity
Mouse CD4
Immunogen
Mouse CTL clone V4
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
PE-Cy7
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
774nm
Ex/Em(nm)
Ex:565nm;Em:774nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD4 Antibody (PE-Cy7) (Ab223928) - Flow Cytometry Flow cytometry analysis of CD4 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with Recombinant CD4 Antibody (PE-Cy7) (Ab223928) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).
Recombinant CD4 Antibody (PE-Cy7) (Ab223928) - Flow Cytometry Flow cytometry analysis of CD4 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with Recombinant CD4 Antibody (PE-Cy7) (Ab223928) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤0.3 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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