GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Recombinant,ExactAb™,Validated,0.5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Recombinant CD5 Antibody, clone P27B11, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD5 Antibody (AF594) - Primary antibody, specific to Mouse CD5
Specifications & Purity
Recombinant, ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance.
Acts by interacting with
Host species
Rat
Specificity
Mouse CD5
Immunogen
Mouse Thymus / Spleen
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
AF594
Excitation (nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD5 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD5 Antibody (AF594) (Ab231912) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF594) (Ab190241).
Flow cytometry analysis of CD5 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD5 Antibody (AF594) (Ab231912) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF594) (Ab190241). Black - Unlabelled control, cells without incubation with primary antibody.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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