Recombinant Core Streptavidin Protein (PE)

Cat. No.: rp230627

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, 1.0 mg/mL

Expression system

E. coli

Bioactivity

Binding Biotin

Overview

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Streptavidin-R-PE conjugate comprises streptavidin (as the biotin-binding protein) with RPE covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry, microplate reader and microarray platforms. This Streptavidin-R-PE product has been tested by immunofluorescent staining with flow cytometric analysis and Multiplex bead-based assay.

Specifications

Product Name

Recombinant Core Streptavidin Protein (PE)

Synonyms

SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin | Streptavidin-R-PE | R-phycoerythrin conjugate | SA-PE | Streptavidin-Phycoerythrin | SAv-PE | RPE-streptavidin conjugate

Grade

Carrier Free, ExactAb™, High Performance, Validated

Specifications & Purity

Carrier Free, ExactAb™, Validated, High Performance, 1.0 mg/mL

Bioactivity

Binding Biotin

Conjugation

PE

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

575nm

Ex/Em(nm)

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Multiplex bead-based assay: Recombinant Core Streptavidin Protein (PE) (rp230627)

Recombinant Core Streptavidin Protein (PE) (rp230627) - Multiplex bead-based assay
Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected.
Capture Antibody (Mouse IgG1): 3 μg/scale
Detection Antibody (Biotin goat Anti-Mouse IgG1): 2500 ng/mL
SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000
Recombinant Core Streptavidin Protein (PE) (rp230627) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis.
Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.

Multiplex bead-based assay: Recombinant Core Streptavidin Protein (PE) (rp230628)

Recombinant Core Streptavidin Protein (PE) (rp230627) - Multiplex bead-based assay
Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected.
Capture Antibody (Mouse IgG1): 3 μg/scale
Detection Antibody (Biotin goat Anti-Mouse IgG1): 10 ng/mL
SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000
Recombinant Core Streptavidin Protein (PE) (rp230627) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis.
Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.

Flow Cytometry: Recombinant Core Streptavidin Protein (PE) (rp230627)

Recombinant Core Streptavidin Protein (PE) (rp230627) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.5μg/mL for 1 hour at 4°C. Then incubated with Recombinant Core Streptavidin Protein (PE) (rp230627) at 0.3μg/mL for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.