GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Pla
Host species
Rat
Specificity
Mouse EpCAM
Immunogen
Glycoconjugates from BALB/c mouse-derived TE-71 medullary thymic epithelial cell line.
Positive Control
Flow Cytometry: 57BL/6 Mouse thymic cells
Conjugation
PE-Cy5
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
670nm
Ex/Em(nm)
Ex:565nm;Em:670nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Multicolor flow cytometric analysis of EpCAM/CD326 expression on live mouse thymocytes. C57BL/6 mouse thymocytes were stained with CD8 alpha Rat mAb (PE) (Ab181177), Recombinant CD4 Antibody (FITC) (Ab223921) and either Rat IgG (PE-Cy5) (Ab190072; Left panel) or Recombinant EpCAM/CD326 Antibody (PE-Cy5) (Ab223774; Right panel) at 0.3 μg/test. A bivariate pseudocolor density plot showing the correlated expression of CD326 (EpCAM) expression (or Isotype control staining) was derived from CD4- gated events with the forward and side light-scatter characteristics of viable thymocytes.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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