GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)
Accession #
Q8N1Q1
Protein Tag
C-10His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions.
ActiBioPure™, Bioactive, Carrier Free, High Performance, His Tag
Specifications & Purity
Carrier Free, Bioactive, ActiBioPure™, High Performance, His-Tag, ≥90%(SDS-PAGE)
Biochemical and Physiological Mechanisms
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible
Bioactivity
Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions.
30.5 kDa, under reducing conditions; 30.5 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Images
Recombinant Human Carbonic Anhydrase XIII Protein (rp183661) - SDS-PAGE 2 μg/lane of Recombinant Human Carbonic Anhydrase XIII Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.5 kDa.
Recombinant Human Carbonic Anhydrase XIII Protein- Enzyme Activity Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions. Conditions: Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5 Recombinant Human Carbonic Anhydrase XIII Protein (rp183661) Substrate: 4-Nitrophenyl Acetate (4-NPA) (N111060), 100 mM stock in acetone 1.Dilute rhCA13 to 100 ng/µL in Assay Buffer. 2.Dilute Substrate to 2.0 mM in Assay Buffer. 3.In a plate load 50 µL of 100 ng/µL rhCA13, and include a Substrate Blank containing 50 µL Assay Buffer. 4.Start the reaction by adding 50 µL of 2.0 mM Substrate to wells containing rhCA13 and Assay Buffer. 5.Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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