GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.Sterile?Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥98%(SDS-PAGE)
Accession #
P02751
Protein Tag
C-His
Expression system
E. coli
Endotoxin Concentration
<10 EU/mg
Bioactivity
It has the effect of promoting cell adhesion. Depending on the cell type, the optimal concentration is 0.2-1.5 μg/cm2
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Fibronectin is a high molecular weight, multi-domain glycoprotein that plays a key role in various cell-cell and cell-matrix processes, such as cell attachment, diffusion, growth, differentiation and migration. It exists on the cell surface and contributes to the ECM structure. This product includes three functional regions: cell binding domain, heparin binding domain and CS1 site. It consists of 574 amino acids and has a molecular weight of 63 kDa. It can greatly improve the infection efficiency of retroviral infection of mammalian cells. Its working principle is: VLA-4 and VLA-5 on the cell surface can bind to the CS-1 site and cell binding domain on this product respectively, and the retroviral vector can bind to the heparin binding domain. On the surface of the culture container coated with this product, cells and viral vectors coexist at high concentrations, thereby improving the efficiency of gene transfection. When coated on the surface of the cell culture dish, the transfection ability of mammalian and insect cells is significantly enhanced.
Specifications
Product Name
Recombinant Human Fibronectin Fragment Protein, CAS No.86088-83-7
ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag, PBS Only, Sterile
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, sterile, His Tag, PBS Only, ≥98%(SDS-PAGE)
Biochemical and Physiological Mechanisms
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compac
Bioactivity
It has the effect of promoting cell adhesion. Depending on the cell type, the optimal concentration is 0.2-1.5 μg/cm2
Reconstitute in water to a concentration of 1 mg/mL, for other concentrations, please adjust the concentration of phosphate in the solution by yourself.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃~-80℃ (2 years). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human Fibronectin Fragment Protein (rp146053) - Protein Bioactivity Fibronectin Fragment Protein has the effect of promoting cell adhesion. Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic fibroblast cells.
Recombinant Human Fibronectin Fragment Protein (rp146053) - SDS-PAGE 2 μg/lane of Recombinant Human Fibronectin Fragment Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
